GB/T 24863-2010 English PDFUS$139.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. GB/T 24863-2010: Technical regulation of farm animals cell culture and cryopreservation Status: Valid
Basic dataStandard ID: GB/T 24863-2010 (GB/T24863-2010)Description (Translated English): Technical regulation of farm animals cell culture and cryopreservation Sector / Industry: National Standard (Recommended) Classification of Chinese Standard: B40 Classification of International Standard: 65.020.30 Word Count Estimation: 6,631 Date of Issue: 2010-06-30 Date of Implementation: 2011-01-01 Quoted Standard: GB/T 6682 Regulation (derived from): National Standard Approval Announcement 2010 No.2 (Total No.157) Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China Summary: This standard specifies the method of preservation of animal cells. This standard applies to pigs, cattle, sheep, horses, asses, chicken, ducks, geese and other poultry in vitro cell culture and preservation. GB/T 24863-2010: Technical regulation of farm animals cell culture and cryopreservation---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.Technical regulation of farm animals cell culture and cryopreservation ICS 65.020.30 B40 National Standards of People's Republic of China Animal and in vitro cell Cryopreservation technical regulations Issued on. 2010-06-30 2011-01-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released ForewordThe standard proposed by the People's Republic of China Ministry of Agriculture. This standard by the National Standardization Technical Committee on Animal Husbandry (SAC/TC274) centralized. This standard was drafted. Beijing Animal Husbandry and Veterinary Research Institute, Chinese Academy of Agricultural Sciences. The main drafters of this standard. Guan Wei Jun, Ma Yuehui, Lixiang Chen, Li Han, Yan Lei, Zhao Qianjun, lotus palace. Animal and in vitro cell Cryopreservation technical regulations1 ScopeThis standard specifies the method to save the livestock body cells. This standard applies to pigs, cattle, sheep, horses, donkeys, chickens, ducks, geese and other poultry in vitro cell culture and preservation.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. Laboratory use specifications and test methods GB/T 6682 Analysis3 Terms and DefinitionsThe following terms and definitions apply to this standard. 3.1 The somatic cells in vitro and in vivo simulated conditions of the living environment in culture, the cells proliferation and retain their structural integrity and functionality The method of characteristics. 3.2 The cells were cultured in a simulated environment in vivo survival, growth and proliferation of the cell culture method can achieve passage states. 3.3 Cultured cells after cryopreservation fluid mixing by a certain percentage, the cooling rate is set by cooling to liquid nitrogen for long term storage of process.4 Workspace conditionsWorkspace generally from the preparation room, buffer room, sterile room, cell preservation chamber. The inter-buffer area should not be less than 3m2, and a locker and ultraviolet light, ultraviolet light intensity is not less than 1.5W/m3. purple Outside the tube from the ground should not exceed 2.5m, each exposure time was 20min ~ 30min. Minimum standards Aseptic sterile room level should reach To ten thousand.5 major instruments and equipmentClean table, refrigerator, oven blast, high-pressure steam sterilizer, liquid nitrogen biological containers, centrifuges, electronic balance, CO2 incubator, Ultra-pure water equipment and microscopes.6 Cleaning and disinfection6.1 glassware cleaning and disinfection 6.1.1 Soak Required glassware containing detergent 清水中浸泡 12h. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 24863-2010_English be delivered?Answer: Upon your order, we will start to translate GB/T 24863-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. 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