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Determination of total ascorbic acid in feeds
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Determination of total ascorbic acid in feeds. o-Phenylenediamine fluorometry
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Basic data
| Standard ID | GB/T 17816-2025 (GB/T17816-2025) |
| Description (Translated English) | Determination of total ascorbic acid in feeds |
| Sector / Industry | National Standard (Recommended) |
| Classification of Chinese Standard | B46 |
| Classification of International Standard | 65.120 |
| Word Count Estimation | 10,121 |
| Date of Issue | 2025-08-01 |
| Date of Implementation | 2026-02-01 |
| Older Standard (superseded by this standard) | GB/T 17816-1999 |
| Issuing agency(ies) | State Administration for Market Regulation, Standardization Administration of China |
GB/T 17816-2025: Determination of total ascorbic acid in feeds
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ICS 65.120
CCSB46
National Standard of the People's Republic of China
Replaces GB/T 17816-1999
Determination of total ascorbic acid in feed
Released on August 1, 2025
Implementation on February 1, 2026
State Administration for Market Regulation
The National Standardization Administration issued
Preface
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting.
This document replaces GB/T 17816-1999 "Determination of total ascorbic acid in feeds - o-phenylenediamine fluorescence method" and GB/T 17816-
Compared with.1999, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
--- Changed the detection limit of o-phenylenediamine fluorescence method and added the limit of quantification (see Chapter 1, Chapter 1 of the.1999 edition);
--- Added high performance liquid chromatography determination method (see Chapter 4);
--- Changed the calculation and presentation of o-phenylenediamine fluorescence analysis results (see 5.5.4, Chapter 8 of the.1999 edition).
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document is proposed and coordinated by the National Feed Industry Standardization Technical Committee (SAC/TC76).
This document was drafted by. Guoliang Wuhan Scientific Research and Design Institute Co., Ltd.
The main drafters of this document are. Shao Rui, Du Yan, Chen Xiyu, Wang Boyuan, Chen Mengying, Ma Shenghui, Wang Zhongyan, Yan Hongyue, Su Yongbo, Qiu Huijuan,
Chang Xiaohong, Wei Shuangzheng, and Liu Xiaomin.
The previous versions of this document and the documents it replaces are as follows.
---First published in.1999 as GB/T 17816-1999;
---This is the first revision.
Determination of total ascorbic acid in feed
1 Scope
This document describes a high performance liquid chromatography and o-phenylenediamine fluorometric method for the determination of total ascorbic acid in feeds.
This document's high performance liquid chromatography method is applicable to the determination of L-ascorbic acid, D-isoascorbic acid in compound feed, concentrated feed, and additive premix feed.
The o-phenylenediamine fluorescence method is applicable to the determination of total ascorbic acid in compound feed, concentrated feed, and additive premix feed.
Determination of.
This document is not applicable to the determination of total ascorbic acid in feeds added in the form of esterified ascorbic acid.
The detection limits of L-ascorbic acid and D-isoascorbic acid by HPLC in this document are both 5 mg/kg, and the quantification limits are both
The detection limit of o-phenylenediamine fluorescence method is 8 mg/kg, and the quantification limit is 25 mg/kg.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 20195 Preparation of animal feed samples
3 Terms and Definitions
There are no terms or definitions that require definition in this document.
4 High-performance liquid chromatography
4.1 Principle
Ascorbic acid in the sample was extracted with metaphosphoric acid solution and determined by high performance liquid chromatography using the external standard method.
4.2 Reagents or materials
Unless otherwise specified, only analytical grade reagents were used.
4.2.1 Water. GB/T 6682, Grade 1.
4.2.2 L-cysteine. high purity.
4.2.3 Potassium dihydrogen phosphate. chromatographically pure.
4.2.4 Hexadecyltrimethylammonium bromide. chromatographically pure.
4.2.5 Phosphoric acid. chromatographically pure.
4.2.6 Methanol. chromatographic grade.
4.2.7 Metaphosphoric acid solution (20 g/L). Weigh 20 g of metaphosphoric acid, dissolve it in water and dilute to 1 L, mix well. Store at 4°C. The expiration date is
1 month.
4.2.8 Trisodium phosphate solution (100 g/L). Weigh 100 g of trisodium phosphate, dissolve it in water and dilute to 1 L, then mix thoroughly.
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