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DNA barcoding screening method - Part 8: Quarantine colletotrichum kahawae
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Basic data Standard ID | SN/T 4877.8-2017 (SN/T4877.8-2017) | Description (Translated English) | DNA barcoding screening method - Part 8: Quarantine colletotrichum kahawae | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | C62 | Word Count Estimation | 8,836 | Date of Issue | 2017-08-29 | Date of Implementation | 2018-04-01 | Regulation (derived from) | National Quality Inspection (2017) 384 | Issuing agency(ies) | General Administration of Customs |
SN/T 4877.8-2017: DNA barcoding screening method - Part 8: Quarantine colletotrichum kahawae---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Screening methods for gene barcodes - Part 8. Quarantine of anthrax)
People's Republic of China entry and exit inspection and quarantine industry standards
Gene barcode screening method
Part 8. Quarantine anthrax
DNAbarcodingscreeningmethod-Part 8. Quarantinecoletotrichumkahawae
Published on.2017-08-29
2018-04-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 4877 "Gene Barcode Screening Method" is divided into 10 parts.
--- Part 1. Quarantine coryneform bacteria;
--- Part 2. Phytophthora xanthan;
--- Part 3. Quarantine phytoplasma;
--- Part 4. Quarantine stem mold;
--- Part 5. Quarantine Pseudomonas spp.;
--- Part 6. Quarantine acidophilus;
--- Part 7. Phytophthora Verticillium;
--- Part 8. Quarantine anthrax;
--- Part 9. Quarantine smut;
--- Part 10. Quarantine Phytophthora.
This part is the eighth part of SN/T 4877.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine, Chinese people
Ningbo Entry-Exit Inspection and Quarantine Bureau of the Republic.
The main drafters of this section. Cheng Yinghui, Zhang Guiming, Gao Ruifang, Wang Ying, Shi Yaqian, Wang Ying, Duan Weijun, Xiang Caiyu, Pan Guang, Wang Hongying,
Xie Xiaolu.
Gene barcode screening method
Part 8. Quarantine anthrax
1 Scope
This part of SN/T 4877 specifies the preface of the barcode screening of the coffee berry anthracnose Colletotrichumkahawae DNA
Amplification, analysis and determination of results.
This section applies to the screening of DNA barcodes for coffee berries anthracnose in imported plants and plant products.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
SN/T 3679 coffee berry anthracnose quarantine identification method
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
DNA barcode DNAbarcode
A standard, sufficiently variable, easily amplified and relatively short DNA fragment of the species that can represent the species.
3.2
Glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase; GAPDH
An enzyme in the glycolysis reaction, with a molecular weight of 146 kDa, is a housekeeping gene and is expressed at high levels in almost all tissues. In the same species
The amount of protein expressed in cells or tissues is generally constant, and is not affected by the inclusion of partial recognition sites, phorbol lipids, etc.
It stays constant while staying.
4 Basic information on coffee berries anthracnose
English name. pathogenofcoffeeberrydisease
Scientific name. ColletotrichumkahawaeJ.M.WaleretBridge
Synonym. Colletotrichumcoffeanumvar.virulans (Rayner, 1952)
Taxonomic status. fungi (Fungi), Anamorphic fungi (Anamorphicfungi), Anthracnose (Colletotrichum).
5 Principle of the method
Using the GAPDH gene as the DNA barcode gene of the coffee berry anthracnose, PCR is performed on the DNA of the test subject
After amplification and sequencing of the product, the NCBI nucleic acid database is used for alignment, and the species are screened according to sequence similarity.
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