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SN/T 4877.8-2017 English PDF

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SN/T 4877.8-2017: DNA barcoding screening method - Part 8: Quarantine colletotrichum kahawae
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Standard similar to SN/T 4877.8-2017

GBZ 57   GB/T 31989   SN/T 4877.14   SN/T 4877.15   SN/T 4877.13   

Basic data

Standard ID SN/T 4877.8-2017 (SN/T4877.8-2017)
Description (Translated English) DNA barcoding screening method - Part 8: Quarantine colletotrichum kahawae
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C62
Word Count Estimation 8,836
Date of Issue 2017-08-29
Date of Implementation 2018-04-01
Regulation (derived from) National Quality Inspection (2017) 384
Issuing agency(ies) General Administration of Customs

SN/T 4877.8-2017: DNA barcoding screening method - Part 8: Quarantine colletotrichum kahawae

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Screening methods for gene barcodes - Part 8. Quarantine of anthrax) People's Republic of China entry and exit inspection and quarantine industry standards Gene barcode screening method Part 8. Quarantine anthrax DNAbarcodingscreeningmethod-Part 8. Quarantinecoletotrichumkahawae Published on.2017-08-29 2018-04-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 4877 "Gene Barcode Screening Method" is divided into 10 parts. --- Part 1. Quarantine coryneform bacteria; --- Part 2. Phytophthora xanthan; --- Part 3. Quarantine phytoplasma; --- Part 4. Quarantine stem mold; --- Part 5. Quarantine Pseudomonas spp.; --- Part 6. Quarantine acidophilus; --- Part 7. Phytophthora Verticillium; --- Part 8. Quarantine anthrax; --- Part 9. Quarantine smut; --- Part 10. Quarantine Phytophthora. This part is the eighth part of SN/T 4877. This part is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine, Chinese people Ningbo Entry-Exit Inspection and Quarantine Bureau of the Republic. The main drafters of this section. Cheng Yinghui, Zhang Guiming, Gao Ruifang, Wang Ying, Shi Yaqian, Wang Ying, Duan Weijun, Xiang Caiyu, Pan Guang, Wang Hongying, Xie Xiaolu. Gene barcode screening method Part 8. Quarantine anthrax

1 Scope

This part of SN/T 4877 specifies the preface of the barcode screening of the coffee berry anthracnose Colletotrichumkahawae DNA Amplification, analysis and determination of results. This section applies to the screening of DNA barcodes for coffee berries anthracnose in imported plants and plant products.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. SN/T 3679 coffee berry anthracnose quarantine identification method

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 DNA barcode DNAbarcode A standard, sufficiently variable, easily amplified and relatively short DNA fragment of the species that can represent the species. 3.2 Glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde-3-phosphate dehydrogenase; GAPDH An enzyme in the glycolysis reaction, with a molecular weight of 146 kDa, is a housekeeping gene and is expressed at high levels in almost all tissues. In the same species The amount of protein expressed in cells or tissues is generally constant, and is not affected by the inclusion of partial recognition sites, phorbol lipids, etc. It stays constant while staying.

4 Basic information on coffee berries anthracnose

English name. pathogenofcoffeeberrydisease Scientific name. ColletotrichumkahawaeJ.M.WaleretBridge Synonym. Colletotrichumcoffeanumvar.virulans (Rayner, 1952) Taxonomic status. fungi (Fungi), Anamorphic fungi (Anamorphicfungi), Anthracnose (Colletotrichum).

5 Principle of the method

Using the GAPDH gene as the DNA barcode gene of the coffee berry anthracnose, PCR is performed on the DNA of the test subject After amplification and sequencing of the product, the NCBI nucleic acid database is used for alignment, and the species are screened according to sequence similarity.

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