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SN/T 4877.7-2017: DNA barcoding screening method--Part 7: Quarantine Verticillium
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DNA barcoding screening method--Part 7: Quarantine Verticillium
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SN/T 4877.7-2017
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Basic data | Standard ID | SN/T 4877.7-2017 (SN/T4877.7-2017) | | Description (Translated English) | DNA barcoding screening method--Part 7: Quarantine Verticillium | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Word Count Estimation | 10,130 | | Date of Issue | 2017-08-29 | | Date of Implementation | 2018-04-01 | | Regulation (derived from) | National Quality Inspection (2017) 384 | | Issuing agency(ies) | General Administration of Customs | SN/T 4877.7-2017: DNA barcoding screening method--Part 7: Quarantine Verticillium---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Screening methods for gene barcodes - Part 7. Verticillium dahliae)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Gene barcode screening method
Part 7. Phytophthora
DNAbarcodingscreeningmethod-Part 7. QuarantineVerticillium
Published on.2017-08-29
2018-04-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 4877 "Gene Barcode Screening Method" is divided into 10 parts.
--- Part 1. Quarantine coryneform bacteria;
--- Part 2. Phytophthora xanthan;
--- Part 3. Quarantine phytoplasma;
--- Part 4. Quarantine stem mold;
--- Part 5. Quarantine Pseudomonas spp.;
--- Part 6. Quarantine acidophilus;
--- Part 7. Phytophthora Verticillium;
--- Part 8. Quarantine anthrax;
--- Part 9. Quarantine smut;
--- Part 10. Quarantine Phytophthora.
This part is part 7 of SN/T 4877.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Institute of Inspection and Quarantine.
Drafters of this section. Gao Ruifang, Zhang Guiming, Wang Ying, Cheng Yinghui, Wang Ying, Lu Xiaoyu, Xiang Caiyu, Pan Guang.
Gene barcode screening method
Part 7. Phytophthora
1 Scope
This part of SN/T 4877 specifies the amplification and analysis of sequences in the DNA barcode screening of Verticillium dahliae and Verticillium dahliae.
And the result is judged, etc.
This section applies to DNA barcode screening of quarantine Verticillium.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 28084 Phytosanitary detection and identification of cotton Verticillium
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
DNA barcode DNAbarcode
A standard, sufficiently variable, easily amplified and relatively short DNA fragment of the species that can represent the species.
3.2
Internal transcribed spacer sequence internaltranscribedspacer; ITS
In eukaryotes, ribosomal DNA consists of a ribosome gene and a spacer adjacent to it, with a genome sequence from 5' to 3'
In order. external transcribed spacer, 18S gene, internal transcribed spacer 1 (ITS1), 5.8S gene, internal transcribed spacer 2 (ITS2),
28S gene and gene spacer sequence. The internal transcribed spacer is the region present between 18SrDNA, 5.8SrDNA and 28SrDNA
The domain, ITS1 and ITS2, as non-coding regions, are less affected by external environmental factors and have a faster evolutionary speed than the coding region.
It is highly conserved among different strains in the species, and there are great changes among the fungi species, showing great sequence polymorphism and can provide
Hermeneutic traits required for detailed systematic analysis.
4 Basic information on quarantine Verticillium
Scientific name. VerticilliumdahliaeKleb
Chinese name. Verticillium dahliae
Scientific name. Verticilliumalbo-atrum ReinkeetBerthold
Chinese name. Verticillium dahliae
Classification status. fungi (Fungi), Hyphomycetes, Hyphomycetales, Monili-
(aceae), Verticillium
See Appendix A for the host range.
5 Principle of the method
Using the ITS fragment as a DNA barcode gene for Verticillium dahliae and Verticillium wilt, through the DNA of the test subject
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