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SN/T 4864-2017: Transgenic maize detection. Taqman array card method
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Transgenic maize detection. Taqman array card method
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SN/T 4864-2017
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Basic data | Standard ID | SN/T 4864-2017 (SN/T4864-2017) | | Description (Translated English) | Transgenic maize detection. Taqman array card method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X11 | | Word Count Estimation | 8,879 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | | Issuing agency(ies) | General Administration of Customs | SN/T 4864-2017: Transgenic maize detection. Taqman array card method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Detection method of micro - fluid microarray for detection of transgenic maize)
People's Republic of China entry and exit inspection and quarantine industry standards
Transgenic corn detection microfluidic chip detection method
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. Publication of this document
The organization does not assume responsibility for identifying these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Liaoning Entry-Exit Inspection and Quarantine Bureau, LifeTechnologiesBrand, Thermofisher
ScientificCorp, Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Xu Junyi, Cao Jijuan, Yalei Wu, Zheng Qiuyue, Xu Yang, Nayong, JennyXiao, He Yan, Wang Jianxin,
Huang Zhi, Chen Dajun.
Transgenic corn detection microfluidic chip detection method
1 Scope
This standard specifies genetically modified corn TC1507, NK603, MON87640, MON863, MON810, MIR162,
17 such as GA21, DAS40278, BT176, BT11, 98140, 59122, 3272, MON89034, MIR604, MON88017, T25, etc.
A microfluidic chip detection method for strains.
This standard applies to genetically modified corn TC1507, NK603, MON87640, MON863, MON810 in corn and its processed products.
MIR162, GA21, DAS40278, BT176, BT11, 98140, 59122, 3272, MON89034, MIR604, MON88017, T25
Qualitative screening tests for 17 lines.
The qualitative detection limit of this method is 1% (mass fraction).
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products
GB/T 19495.3 Transgenic product detection nucleic acid extraction and purification method
GB/T 19495.7 GM product testing sampling and sample preparation method
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Transgenic transgene
a functional DNA sequence derived from other species that is not native to the species, and which is introduced into the species through various means of introduction.
Line expression so that the species acquires new cultivar characteristics.
3.2
Endogenous gene
A gene with a constant copy number in the cultivated species that does not show allelic changes. This gene can be used for a purpose in the genome
Quantitative analysis of genes.
3.3
Exogenous gene
Other biological genes transferred using bioengineering techniques allow the organism to exhibit new biological traits.
4 Principle of the method
TaqMan technology is a patented technology applied to real-time PCR detection of nucleic acids. This technique uses synthetic design based on target nucleic acid
The DNA is single-stranded and coupled to a fluorescent reporter group and a fluorescence quenching group at both ends of the single strand, and this structure is called a TaqMan probe.
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