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Identification of common honey plant species in export honey. Real-time PCR method - Part 4: Eucalyptus
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Basic data Standard ID | SN/T 4848.4-2017 (SN/T4848.4-2017) | Description (Translated English) | Identification of common honey plant species in export honey. Real-time PCR method - Part 4: Eucalyptus | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | X31 | Word Count Estimation | 9,974 | Date of Issue | 2017-07-21 | Date of Implementation | 2018-03-01 | Quoted Standard | GB/T 6682; GB/T 27403-2008 | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | Issuing agency(ies) | General Administration of Customs | Summary | This standard specifies the real-time fluorescent PCR detection method for eucalyptus components in exported honey. This standard applies to the qualitative detection of eucalyptus components (lemon scorpion, blue scorpion, giant scorpion, straight sorghum, big leaf scorpion, scorpion scorpion scorpion, red peony) in exported honey. The minimum detection limit (LOD) of the method specified in this standard is 0.5% (mass percent). |
SN/T 4848.4-2017: Identification of common honey plant species in export honey. Real-time PCR method - Part 4: Eucalyptus ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of common honey plant species in export honey.Real-time PCR method - Part 4. Eucalyptus
People's Republic of China entry and exit inspection and quarantine industry standards
Method for detecting common honey plant components in exported honey
Real-time fluorescent PCR
Part 4. Eucalyptus
Part 4. Eucalyptus
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 4848 "Reagents for the detection of common honey plant components in honey. Real-time fluorescent PCR method" series of standards are divided into 8 parts.
--- Part 1. Vitex;
--- Part 2. Rape;
--- Part 3. Artichoke;
--- Part 4. Eucalyptus;
--- Part 5. Eucalyptus;
--- Part 6. Longan;
--- Part 7. Lychee and Longan;
--- Part 8. Zin Yunying.
This part is part 4 of the SN/T 4848.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. China Academy of Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people
And the National Anhui Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this section. Li Xiang, Han Li, Xue Huajie, Li Hui, Ren Yifei, Wu Yajun, Zong Kai, Chen Ying, Zheng Yi, Men Guangqi, Yang Yange,
Han Jianxun.
Method for detecting common honey plant components in exported honey
Real-time fluorescent PCR
Part 4. Eucalyptus
1 Scope
This part of SN/T 4848 specifies a real-time fluorescent PCR assay for the extraction of eucalyptus components from honey.
This section applies to the qualitative inspection of eucalyptus components (lemon scorpion, blue scorpion, giant scorpion, straight sorghum, big leaf scorpion, scorpion scorpion, red peony) in exported honey.
Measurement. The minimum detection limit (LOD) for the method specified in this section is 0.5% (mass percent).
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 27403-2008 Laboratory quality control regulations Food molecular biology testing
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Honey honey
The bee collects the nectar, secretions or honeydew of the plant, and mixes it with its own secretions, and then fully brews the natural sweet substance.
3.2
Eucalyptus eucalyptus
The eucalyptus is a myrtaceae plant with a flower-like shape and a pink color.
3.3
Real-time fluorescent PCR realtimePCR
Adding a fluorophore to the PCR reaction system and monitoring the entire PCR amplification process in real time using the accumulation of fluorescent signals to achieve the initial
Quantitative and qualitative analysis of the template.
3.4
Ct value cyclethreshold
The number of cycles experienced by the fluorescent signal in each reaction tube when it reaches the set domain value.
4 Abbreviations
The following abbreviations apply to this document.
DNA. deoxyribonuleic acid
PCR. polymerase chain reaction (polymerase chain reaction)
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