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SN/T 4848.1-2017

Chinese Standard: 'SN/T 4848.1-2017'
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SN/T 4848.1-2017English199 Add to Cart Days<=3 Identification of common honey plant species in export honey. Real-time PCR method - Part 1: Chaste tree Valid SN/T 4848.1-2017
SN/T 4848.1-2017Chinese16 Add to Cart <=1-day [PDF from Chinese Authority, or Standard Committee, or Publishing House]

   

BASIC DATA
Standard ID SN/T 4848.1-2017 (SN/T4848.1-2017)
Description (Translated English) Identification of common honey plant species in export honey. Real-time PCR method - Part 1: Chaste tree
Sector / Industry Commodity Inspection Standard (Recommended)
Word Count Estimation 9,935
Date of Issue 2017-07-21
Date of Implementation 2018-03-01
Quoted Standard GB/T 6682; GB/T 14963-2011; GB/T 27403-2008
Drafting Organization China Academy of Inspection and Quarantine
Administrative Organization National Certification and Accreditation Administration
Regulation (derived from) State-Quality-Inspection-Accreditation (2017) 337
Proposing organization National Certification and Accreditation Administration
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the PRC
Summary This standard specifies the real-time fluorescent PCR detection method for the components in the honey. This standard applies to the qualitative detection of the ingredients in the export honey. The minimum detection limit (LOD) of the method specified in this standard is 0.5% (mass percent).

SN/T 4848.1-2017
Identification of common honey plant species in export honey.Real-time PCR method - Part 1. Chaste tree
People's Republic of China entry and exit inspection and quarantine industry standards
Method for detecting common honey plant components in exported honey
Real-time fluorescent PCR
Part 1. Vitex
Part 1. Chastetree
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 4848 "Reagent Method for Detection of Common Honey Plant Components in Exported Honey" is divided into 8 parts.
--- Part 1. Vitex;
--- Part 2. Rape;
--- Part 3. Artichoke;
--- Part 4. Eucalyptus;
--- Part 5. Eucalyptus;
--- Part 6. Longan;
--- Part 7. Lychee and Longan;
--- Part 8. Zin Yunying.
This part is the first part of SN/T 4848.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. China Academy of Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this section. Chen Ying, Yang Yange, Wu Yajun, Han Jianxun, Huang Wensheng, Zhang Jiukai, Wang Bin, Li Xiang, Liu Mingchang.
Method for detecting common honey plant components in exported honey
Real-time fluorescent PCR
Part 1. Vitex
1 Scope
This part of SN/T 4848 specifies a real-time fluorescent PCR assay for the extraction of Vitex in honey.
This section applies to the qualitative detection of the ingredients in the export honey. The minimum detection limit (LOD) for the method specified in this section is 0.5%
(% by mass).
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 14963-2011 National Food Safety Standard Honey
GB/T 27403-2008 Laboratory quality control regulations Food molecular biology testing
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Honey honey
The bee collects the nectar, secretions or honeydew of the plant, and mixes it with its own secretions, and then fully brews the natural sweet substance.
3.2
Vitex chastetree
Vitexnegundo L.var.heterophyla (Franch.) Rehd., also known as Vitex, Huangjing Chai, Golden Son, Verbenaceae,
Vitex, belonging to the genus Verbena.
3.3
Real-time fluorescent PCR realtimePCR
Adding a fluorophore to the PCR reaction system and monitoring the entire PCR amplification process in real time using the accumulation of fluorescent signals to achieve the initial
Quantitative and qualitative analysis of the template.
3.4
Ct value cyclethreshold
The number of cycles experienced by the fluorescent signal in each reaction tube when it reaches the set domain value.
4 Abbreviations
The following abbreviations apply to this document.
Related standard: SN/T 4848.2-2017    SN/T 4848.3-2017
Related PDF sample: GB/T 317-2018    GB/T 33045-2016