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SN/T 4807-2017: Determination of bacitracin in animals and feeds for import and export
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4807-2017 | English | 209 |
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Determination of bacitracin in animals and feeds for import and export
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SN/T 4807-2017
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Basic data | Standard ID | SN/T 4807-2017 (SN/T4807-2017) | | Description (Translated English) | Determination of bacitracin in animals and feeds for import and export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B46 | | Word Count Estimation | 9,929 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 337 | | Issuing agency(ies) | General Administration of Customs | SN/T 4807-2017: Determination of bacitracin in animals and feeds for import and export---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Import and export of edible animals, feeding bacitracin detection method)
People's Republic of China entry and exit inspection and quarantine industry standards
Import and export of food animals, feed bacitracin
Detection method
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Zhang Xiaowen, Zhang Xiaomei, Zheng Xiaolong, Liu Jingjing, Wang Fengmei, Wang Qun, Wang Peifeng, Zhang Jinling.
Import and export of food animals, feed bacitracin
Detection method
1 Scope
The first method of this standard stipulates the liquid chromatography-tandem mass spectrometry method for the determination of bacitracin A in the blood and feed of imported and exported food animals, and the second method.
The law stipulates an ELISA method for the detection of bacitracin in food animals and feeds.
This standard applies to the determination of bacitracin in the blood and feed of imported and exported food animals (pig, cattle, sheep, poultry).
This standard applies to entry and exit inspection and quarantine work.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
Method for detection of bacitracin A in blood and feed of foods imported and exported by the first method, liquid chromatography tandem mass spectrometry
3 method summary
After the sample is pulverized and homogenized, the residue of bacitracin A in the sample is extracted with a methanol-formic acid aqueous solution, and purified by a solid phase extraction cartridge.
Chromatography-tandem mass spectrometry, external standard method for quantification.
4 reagents
Unless otherwise stated, the reagents used were of analytical grade and the water was the first grade water specified in GB/T 6682.
4.1 Dichloroacetic acid.
4.2 EDTA.
4.3 TCA.
4.4 Methanol. Chromatographically pure.
4.5 Acetonitrile. Chromatographically pure.
4.6 Formic acid. chromatographically pure.
4.7 1.5mmol/LEDTA solution. Weigh 0.5g EDTA into a volumetric flask and dilute to 1000mL with water.
4.8 1% TCA solution. Weigh 10.0g of TCA in a volumetric flask and dilute to 1000mL with water.
4.9 0.1% formic acid-water solution. Pipette 1 mL of formic acid into a volumetric flask and dilute to 1000 mL with water.
4.10 4% trichloroacetic acid-acetonitrile solution. Pipette 4 mL of dichloroacetic acid in a volumetric flask and make up to 100 mL of acetonitrile.
4.11 Constant volume solution. 0.1% formic acid-water solution acetonitrile volume ratio of 8.2.
4.12 Bacitracin A standard. purity ≥ 96.0%.
4.13 Standard stock solution. Accurately weigh the bacitracin standard, dissolve to a volume of 10 mL with methanol (4.3), and the solution concentration is 1000 μg/mL.
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