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SN/T 4784-2017: Qualitative detection of Norovirus and Hepatitis A virus in food for export. Real-time RT-PCR
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Qualitative detection of Norovirus and Hepatitis A virus in food for export. Real-time RT-PCR
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SN/T 4784-2017
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Basic data | Standard ID | SN/T 4784-2017 (SN/T4784-2017) | | Description (Translated English) | Qualitative detection of Norovirus and Hepatitis A virus in food for export. Real-time RT-PCR | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 13,116 | | Date of Issue | 2017-05-12 | | Date of Implementation | 2017-12-01 | | Regulation (derived from) | National Quality Inspection (2017) 228 | | Issuing agency(ies) | General Administration of Customs | SN/T 4784-2017: Qualitative detection of Norovirus and Hepatitis A virus in food for export. Real-time RT-PCR
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Real - time RT - PCR method for detection of Norovirus and Hepatitis A virus in foodstuffs)
People's Republic of China entry and exit inspection and quarantine industry standards
Norovirus and hepatitis A virus in exported foods
Detection method real-time RT-PCR method
Released on.2017-05-12
2017-12-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Yantai Entry-Exit Inspection and Quarantine
Bureau, Dandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Zhao Yuran, Yin Weili, Xue Xiaoning, Yue Zhiqin, Fang Baohai, Zhang Wei, Ma Lidan, Liu Xin, Zheng Xiaolong, Sun Tao,
Wang Qun.
Norovirus and hepatitis A virus in exported foods
Detection method real-time RT-PCR method
1 Scope
This standard specifies real-time fluorescent RT-PCR of norovirus GI, GII and hepatitis A virus in soft fruit, hard fruit, bottled water and shellfish.
Detection method.
This standard applies to the qualitative detection of norovirus GI, GII type and hepatitis A virus in soft fruit, hard fruit, bottled water and shellfish.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products
3 Terms and definitions and abbreviations
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1
Norovirus Norovirus
Norovirus is a single-stranded positive-strand RNA virus belonging to the genus Calicivirus, a virus that causes non-bacterial acute gastroenteritis.
Norovirus is highly infectious, mainly transmitted by the intestines. It is transmitted through the mouth-dung and is mainly transmitted by polluted water, food, shellfish, articles and air.
Broadcast carrier. Norovirus can be divided into five gene groups of GI~GV, among which GI and GII groups are mainly caused by human infection.
3.1.2
Hepatitis A virus Hepatitis Avirus
Hepatitis A virus is a single-stranded positive-strand RNA virus, and in the microRNA virus family, hepadnavirus is a genus, and this genus is only one species of HAV.
After human infection with HAV, most of them manifest as subclinical or latent infection, vomiting, diarrhea, jaundice, and the virus mainly through fecal-oral
The path is transmitted by polluted water sources, food, seafood (such as buttercups), utensils, etc. as a carrier of transmission.
3.1.3
Ct value cyclethresholdvalue
The number of cycles experienced when the fluorescent signal reaches a set threshold.
3.1.4
Process quality control material processcontrolmaterial
Monitor the entire assay by adding a known, known amount of exogenous control material similar to the virus of interest, including virus collection, nucleic acid extraction
The real-time fluorescence RT-PCR process was used to evaluate the effectiveness of the entire detection process by calculating the recovery rate of the quality control material in the final process.
3.1.5
Exogenous control RNA external controlRNA
Adding a known level of exogenous control similar or identical to the RNA of interest during real-time fluorescent RT-PCR
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