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Determination of kestose, nistose and fructofuranosylnystose in export food--High performance liquid chromatography method
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SN/T 4783-2017
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Basic data Standard ID | SN/T 4783-2017 (SN/T4783-2017) | Description (Translated English) | Determination of kestose, nistose and fructofuranosylnystose in export food--High performance liquid chromatography method | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | X04 | Word Count Estimation | 18,114 | Date of Issue | 2017-05-12 | Date of Implementation | 2017-12-01 | Regulation (derived from) | State-Quality-Inspection-Accreditation (2017) 228 | Issuing agency(ies) | General Administration of Customs |
SN/T 4783-2017: Determination of kestose, nistose and fructofuranosylnystose in export food--High performance liquid chromatography method ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Determination of oligosaccharide fructose in low-fructose, low fructose and low fructose in export food. High performance liquid chromatography)
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Low-polytriosin, oligo-fructose,
Determination of oligo-5-fructose by high performance liquid chromatography
Released on.2017-05-12
2017-12-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, international inspection and quarantine standards and technical regulations in the study
Heart, Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, South China Agricultural University.
The main drafters of this standard. Wei Xiaoqun, Zheng Yi, Song Zhigang, Yan Tingting, Du Sihang, Wu Zhihang, Zhang Yuwen, Huang Weilong, Chen Wenrui, Liu Qing,
Yi Rong, Wang Zhiyuan, Liang Ruiting, Lu Pearl.
Low-polytriosin, oligo-fructose,
Determination of oligo-5-fructose by high performance liquid chromatography
1 Scope
This standard stipulates the determination of three kinds of oligofructose, such as infant formula rice flour, rice milk powder and low-poly cane fruit trisaccharide, tetrasaccharide and pentasaccharide in milk powder.
Method.
This standard is applicable to the determination of low poly cane fruit trisaccharide, tetrasaccharide and pentasaccharide in infant formula rice flour, rice milk powder and milk powder.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Method summary
After precipitation of protein and starch by precipitation of acetic acid water and methanol solution at a given pH, the oligofructose in the sample is then treated with β-galactosidase.
Remove galactose and lactose, pass the membrane test, and quantify by external standard method.
4 reagents and materials
Unless otherwise stated, the reagents used were of analytical grade and the water used secondary water specified in GB/T 6682.
4.1 Methanol (CH4O), analytically pure.
4.2 Acetic acid (C2H4O2), analytically pure.
4.3 Acetonitrile (C2H3N), chromatographically pure.
4.4 Disodium hydrogen phosphate (Na2HPO4), analytically pure.
4.5 Citric acid (C6H8O7), analytically pure.
4.6 oligofructose standard. canetriose (C18H32O16, CASN o.. 470-69-9), cane fruit tetrasaccharide (C24H42O21, CASN o..
13133-07-8), cane fruit pentasaccharide (C30H52O26, CASN o.. 59432-60-9) three standards, purity ≥ 99%.
4.7 Cane fruit trisaccharide, cane fruit tetrasaccharide, cane fruit and pentasaccharide mixed standard stock solution. weigh the cane, fructose, sugar, sugar, cane and fruit
2.5g of the standard product, dissolved together in water and fixed to a 50mL volumetric flask, and prepared into a sugar cane trisaccharide and cane fruit with a concentration of 50000mg/mL respectively.
A standard solution of sugar, cane and fruit sugar mixed, stored at 2 ° C ~ 4 ° C, valid for 3 months.
4.8 Standard intermediate solution of cane fructose, cane fruit tetrasaccharide and cane fruit and pentasaccharide. accurately measure the single standard solution of 50000mg/mL (4.7)
5.0mL, fixed to 25mL with water, formulated into a concentration of 10000mg/mL of cane fructose, cane fruit four sugar, cane fruit and five sugar mixed standard
Solution.
4.9 Candied fruit trisaccharide, cane fruit tetrasaccharide, cane fruit and pentasaccharide standard mixed working solution. separately transfer 10000mg/mL cane fructose, tetrasaccharide, pentasaccharide
Standard intermediate solution (4.8) 5mL, 2.5mL, 1mL, 0.5mL, 0.1mL, with pure water to a volume of 10mL into 5000mg/mL,
2500mg/mL, 1000mg/mL, 500mg/mL, 100mg/mL standard working solution, stored at 4 ° C, can be used for 3 months.
4.10 1mol/L disodium hydrogen phosphate buffer with a pH of 5.0. first weigh 1.92g of citric acid and dissolve it in 10mL of pure water.
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