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Detection and identification of Gremmeniella abietina(Lagerberg) Morelet
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SN/T 4731-2016
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Basic data | Standard ID | SN/T 4731-2016 (SN/T4731-2016) | | Description (Translated English) | Detection and identification of Gremmeniella abietina(Lagerberg) Morelet | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 12,153 | | Date of Issue | 2016-12-12 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.590 | | Issuing agency(ies) | General Administration of Customs | SN/T 4731-2016: Detection and identification of Gremmeniella abietina(Lagerberg) Morelet---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Gremmeniella abietina (Lagerberg) Morelet
People's Republic of China Entry - Exit Inspection and Quarantine Industry Standard
Quarantine Identification Method of Abies
2016-12-12 release
2017-07-01 implementation
People's Republic of China
State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and approved by the National Certification and Accreditation Administration Committee.
The drafting unit of this standard. the People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, the People's Republic of China Shanghai Entry-Exit Inspection and Quarantine
Bureau of the People's Republic of China Shenzhen Entry - Exit Inspection and Quarantine Bureau, the People' s Republic of China Tianjin Entry - Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Li Bin, Su Han, Jiao Binbin, Wang Ying, Li Xiaoqi, Wu Cuiping, An Yulin.
Quarantine Identification Method of Abies
1 Scope
This standard specifies the method for the identification of fecal firming bacteria (Gremmeniellaabietina).
This standard applies to the quarantine of fir fir shoots in host plants such as fir, larch, spruce and pine and their plant products
Identification.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
Sampling method for quarantine of inbound and outbound plants and plant products
3 basic information
Scientific name. Gremmeniellaabietina (Lagerberg) Morelet.
Unaccompanied. Brunchorstiapinea (P. Karsten) Höhnel.
Taxonomic status. Fungi, Ascomycota, Ascomycetes, Helotia-
les, Helotiaceae, Gremmeniella.
For reference, see Appendix A.
4 principle
The culture traits, morphological characters and molecular biological characteristics of Abies faxoniana were the main basis for the identification of the pathogen.
5 Instruments, appliances and reagents
5.1 Major equipment and equipment
Biological microscope, stereomicroscope, clean bench, sterilization pot, incubator, ultra-low temperature refrigerator, conventional refrigerator, vortex oscillator, high-speed freezing
Desktop centrifuge, pure water meter, PCR instrument, fluorescence quantitative PCR instrument, electrophoresis instrument, gel imaging system, water bath, balance (inductance. 0.001g) and so on.
5.2 Main appliances
Sample bags, labels, magnifying glass, petri dishes, slides, coverslips, triangular bottles, test tubes, beakers, scalpels, surgical scissors, tweezers, mortar,
(2 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL), various pipettes,
(0.2 μl, 0.5 mL), a centrifuge tube (0.2 mL, 0.6 mL, 1.5 mL), and the like (10 μL,.200 μL, 1000 μL).
5.3 Main reagents and media
Unless otherwise specified, all reagents are analytical or biochemical reagents, the experimental water in line with GB/T 6682.
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