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SN/T 4661-2016 English PDF

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SN/T 4661-2016: Quarantine protocol for Schmallenberg disease
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PDF similar to SN/T 4661-2016


Standard similar to SN/T 4661-2016

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Basic data

Standard ID SN/T 4661-2016 (SN/T4661-2016)
Description (Translated English) Quarantine protocol for Schmallenberg disease
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Word Count Estimation 14,111
Date of Issue 2016-12-12
Date of Implementation 2017-07-01
Regulation (derived from) State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies) General Administration of Customs

SN/T 4661-2016: Quarantine protocol for Schmallenberg disease

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Technical specification for quarantine of Schmallenberg disease) People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Schmallenberg disease quarantine technical specification Released on December 12,.2016 2017-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. China Academy of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the Chinese people And the Liaoning Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Zhang Yongning, Wu Shaoqiang, Lin Xiangmei, Wang Na, Lu Jizhou, Feng Chunyan, Deng Junhua, Wang Jianchang, Hu Chuanwei. Schmallenberg disease quarantine technical specification

1 Scope

This standard specifies the technique of real-time fluorescence reverse transcription-polymerase chain reaction and blocking enzyme-linked immunosorbent assay for Schmallenberg disease. specification. This standard applies to the quarantine of Schmallenberg disease.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 18088 entry and exit animal quarantine sampling GB 19489 General requirements for laboratory biosafety

3 Abbreviations

The following abbreviations apply to this document. SBV. Schmalenberg virus RNA. Ribonucleic acid rRT-PCR. real-time fluorescence reverse transcription-polymerase chain reaction (Real-timereversetranscriptionpolymerasechainre- Action) Ct value. Cycle threshold (Cyclethreshold) ELISA. Enzyme linked immunosorbent assay McAb. monoclonal antibody (Monoclonalantibody) TBST. Contains 0.05% Tween-20 Tris-HCl buffer (Tris-bufferedsalinecontaining0.05% Tween20) OD value. optical density value (Optical density value) PI. Blocking rate (Percentageofinhibition)

4 Schmallenberg disease overview

See Appendix A for an overview of Schmallenberg disease. 5 Real-time fluorescence reverse transcription-polymerase chain reaction (rRT-PCR) 5.1 Main reagents and materials 5.1.1 TRIzol reagent. 5.1.2 No RNase water. 5.1.3 SBVRNA negative control, SBVRNA positive control. A pseudovirus containing the SBVN gene was provided by a standard drafting unit.

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