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SN/T 4660-2016: Identification technology protocol for deer species
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| SN/T 4660-2016 | English | 229 |
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Identification technology protocol for deer species
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SN/T 4660-2016
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Basic data | Standard ID | SN/T 4660-2016 (SN/T4660-2016) | | Description (Translated English) | Identification technology protocol for deer species | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 10,189 | | Date of Issue | 2016-12-12 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | State-Quality-Inspection-Accredidation (2016) No.590 | | Issuing agency(ies) | General Administration of Customs | SN/T 4660-2016: Identification technology protocol for deer species---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification technology protocol for deer species
China's entry-exit inspection and quarantine industry standards
Deer species identification specification
Published on.2016-12-12
2017-07-01 Implementation
China
The State Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission.
This standard was drafted by. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Institute of Special Products of the Chinese Academy of Agricultural Sciences, Jilin Agriculture
University, Jilin Province, China-Korea Animal Science Institute.
The main drafters of this standard. Jia Wei, Yan Shen Shen, Sun Mingying, Wang Quankai, Zhang Xue, Song Dahe, Wang Kaiying, Xu Chao, Hu Chuanwei, Yuan Wenze.
Deer species identification specification
1 Scope
This standard specifies the PCR identification method for spot deer and red deer species.
This standard applies to species identification of spotted deer, red deer, and their products.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
3 Methodology
According to the published mitochondrial genome sequences of the deer and red deer in the GenBank database, they are conserved within the species and interspecies
Select specific regions with specificity, design and synthesize 3 primers. After the sample has been extracted from the total genomic DNA, use special design features.
The heterologous primers were used for PCR amplification of the target genes of spotted deer and red deer, and whether the samples contained spotted deer were determined based on the results of PCR amplification.
Red deer gene.
4 Morphological features
Deer and red deer morphological characteristics see Appendix A
5 PCR detection
5.1 Major Instruments and Equipment
PCR machines, tissue grinders, ultra-clean benches, ice makers, high-speed refrigerated centrifuges, water baths, bench top centrifuges, conventional refrigerators,
Vortex oscillator, electrophoresis apparatus, gel imaging system, micro-adjustable pipette (10 μL, 100 μL, 1000 μL) and matching tips.
5.2 Reagents and Materials
Unless otherwise specified, the reagents used in this standard are of analytical grade, and the test water should meet the requirements of GB/T 6682.
5.2.1 anhydrous ethanol.
5.2.2 distilled water (should be in line with the specifications of the first-grade water in GB/T 6682).
5.2.3 Protease K (20 mmol/μL).
5.2.4 10% sodium dodecyl sulfate (SDS).
5.2.5 Cetyltriethylammonium bromide (CTAB).
5.2.6 Sodium chloride.
5.2.7 Trichloromethane.
5.2.8 Isopropyl alcohol.
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