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SN/T 4103-2015: Method of polymerase chain reaction for detecting genetically modified pigs and their derived products
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Method of polymerase chain reaction for detecting genetically modified pigs and their derived products
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SN/T 4103-2015
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Basic data | Standard ID | SN/T 4103-2015 (SN/T4103-2015) | | Description (Translated English) | Method of polymerase chain reaction for detecting genetically modified pigs and their derived products | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X22 | | Classification of International Standard | 67.120.10 | | Word Count Estimation | 10,180 | | Date of Issue | 2015-02-09 | | Date of Implementation | 2015-09-01 | | Quoted Standard | GB/T 6682; GB/T 21101 | | Regulation (derived from) | State-Quality-Inspection-Accreditation [2015] 59 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the transgenic pigs and processed GMO products Qualitative PCR detection method. This standard applies to transfer ��-3 fatty acid desaturase enzyme, phytase and knock the qualitative detection of myostatin gene and its processing products in pig genetically modified ingredients. | SN/T 4103-2015: Method of polymerase chain reaction for detecting genetically modified pigs and their derived products
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Qualitative PCR Detection and processed pig products, GMO)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Swine and processed products in qualitative GMO
PCR detection method
Issued on. 2015-02-09
2015-09-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Beijing Institute of Animal Chinese Academy of Agricultural Sciences, the People's Republic of China
State Shandong CIQ.
The main drafters of this standard. Lin Xiangmei, Wang Qin, Wang Na, Liu, Zhu Zhen camp, Li Kui, Pandeng Ke, Sun Min, Fu Wei, Chousong Yin, Li Xiaolin.
Swine and processed products in qualitative GMO
PCR detection method
1 Scope
This standard specifies the transgenic pigs and processed GMO products Qualitative PCR detection method.
This standard applies to transfer ω-3 fatty acid desaturase enzyme, phytase and knockout swine myostatin gene and its processed products transit
Qualitative detection of gene components.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 21101 animal feed pigs derived materials PCR method
3 Terms, definitions and abbreviations
The following abbreviations, terms and definitions apply to this document.
3.1 Terms and Definitions
3.1.1
Transgenic transgene
This species does not have the will, from other species of functional DNA sequences, by introducing a variety of means to carry out the species
Expression, in order for the species to obtain new varieties of features.
3.2 Acronyms
β2M. β2-microglobulin (β2-microglobulin).
MSTN. Myostatin (myostatin).
Sfat-1. ω-3 fatty acid desaturase (omega-3fattyaciddesaturase).
APPA. phytase (phytase).
mtDNA. Swine DNA (mitochondrialDNA).
TE. Tris · Cl, EDTA buffer.
Tris. Tris (hydroxymethyl) aminomethane (tris (hydroxymethyl) aminomethane).
4 Reagents
4.1 PCR using primers
Internal reference gene (pig mitochondrial DNA) primers were designed with reference to GB/T 21101, within the reference gene (pig β2M gene) exogenous gene primers
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