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SN/T 4065-2014 English PDF

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SN/T 4065-2014: Determination of methyl hydrogen fumarate and dimethyl fumarate in botanic Chinese medicinal material for export - Liquid chromatography method
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SN/T 4065-2014English449 Add to Cart 4 days [Need to translate] Determination of methyl hydrogen fumarate and dimethyl fumarate in botanic Chinese medicinal material for export - Liquid chromatography method Valid SN/T 4065-2014

PDF similar to SN/T 4065-2014


Standard similar to SN/T 4065-2014

GB/T 41907   SN/T 4062   SN/T 4063   SN/T 4061   

Basic data

Standard ID SN/T 4065-2014 (SN/T4065-2014)
Description (Translated English) Determination of methyl hydrogen fumarate and dimethyl fumarate in botanic Chinese medicinal material for export - Liquid chromatography method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C23
Classification of International Standard 11.120.10
Word Count Estimation 17,174
Date of Issue 12/1/2014
Date of Implementation 5/1/2015
Quoted Standard GB/T 6682
Regulation (derived from) State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the method for liquid chromatography vegetable exports of Chinese herbal medicines in monomethyl fumarate and residual amounts of dimethyl fumarate. This standard is applicable to the export of plant Schisandra Chinese herbal medic

SN/T 4065-2014: Determination of methyl hydrogen fumarate and dimethyl fumarate in botanic Chinese medicinal material for export - Liquid chromatography method


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(Export plant in herbal medicines monomethyl fumarate and dimethyl fumarate Determination of residual liquid chromatography) People's Republic of China Entry-Exit Inspection and Quarantine Standards Exports of Chinese herbal medicines in plant and monomethyl fumarate Determination of dimethyl fumarate residues Liquid chromatography Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Chongqing, People's Republic of China Exit Inspection and Quarantine, People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Tang Bobin, Caoshu Rui, Zheng Xiaoling, Deng Xiaojun, was kept Xi, Zhang Lei, Wang Guomin. Exports of Chinese herbal medicines in plant and monomethyl fumarate Determination of dimethyl fumarate residues Liquid chromatography

1 Scope

This standard specifies the method for liquid chromatography vegetable exports of Chinese herbal medicines in monomethyl fumarate and residual amounts of dimethyl fumarate. This standard is applicable to the export of plant Schisandra Chinese herbal medicines, dried tangerine peel, Chinese wolfberry, bitter almonds, cassia, dogwood, cardamom and Arctium of fumaric Determination of monomethyl maleate and dimethyl fumarate residues.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Method summary

Sample monomethyl fumarate, dimethyl fumarate and extracted with ethyl acetate, the extract was centrifuged, filtered, concentrated, dried amino compound stone Ink carbon column solid phase extraction separation, purification, eluent was concentrated and after constant volume, measured by HPLC, external standard.

4 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade water as a water line with GB/T 6682 regulations. 4.1 Methanol. chromatographically pure. 4.2 ethyl acetate. chromatography. 4.3 glacial acetic acid. Chromatographic pure. 4.4 phosphoric acid. 4.5 anhydrous sodium. 650 ℃ burning 4h, cooled in a desiccator to room temperature and stored in a sealed bottle aside. 4.6 0.05% phosphoric acid. Pipette 0.5mL phosphate, was diluted with water to 1000mL. 4.7 HPLC mobile phase. methanol and 0.05% phosphoric acid (3565, volume ratio). 4.8 Glacial acetic acid - ethyl acetate solution (298, volume ratio). Pipette 2mL of glacial acetic acid was diluted to 100mL with ethyl acetate. 4.9 Standards and standard stock solution. monomethyl fumarate standards (Methylhydrogenfumarate, C5H6O4, CAS No. 2756- 87-8). purity greater than or equal to 99%; dimethyl fumarate standards (Dimethylfumarate, C6H8O4, CAS No. 624-49-7). purity Greater than or equal to 98%. Accurately weighed amount of standard, prepared in methanol and 1000μg/mL stock solution of a single standard, 0 ℃ ~ 4 ℃ save. 4.10 mixed intermediate standard solution (10μg/mL). Pipette 1.00mL stock solution of monomethyl fumarate and dimethyl fumarate stock standard separately Solution (4.9) in 100mL flask, diluted with methanol and set the volume to the mark, 0 ℃ ~ 4 ℃ preservation. 4.11 mixed standard working solution. before use take appropriate 10μg/mL mixed intermediate standard solution (4.10), phase (4.7) was diluted with a flow

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