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SN/T 3965-2014 English PDF

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SN/T 3965-2014English479 Add to Cart 3 days [Need to translate] Detection and identification of Erwinia persicina Hao et al. Obsolete SN/T 3965-2014

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Basic data

Standard ID SN/T 3965-2014 (SN/T3965-2014)
Description (Translated English) Detection and identification of Erwinia persicina Hao et al.
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 07.080
Word Count Estimation 12,176
Date of Issue 4/9/2014
Date of Implementation 11/1/2014
Quoted Standard GB/T 6682; SN/T 1809
Regulation (derived from) State inspection certification (2014) 210
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the export plant quarantine peach Erwinia quarantine and identification methods. This standard applies to possible quarantine and identification of Erwinia carry peach fruit, seeds and seedlings and other propagation materials.

SN/T 3965-2014: Detection and identification of Erwinia persicina Hao et al.

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(Peach quarantine and identification of Erwinia) People's Republic of China Entry-Exit Inspection and Quarantine Standards Peach quarantine and identification of Erwinia Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Guangdong Entry Inspection and Quarantine Bureau, Jiangxi Agricultural University. The main drafters of this standard. Feng Lixia, Cuiru Jiang, what Terry, head Wu Tao, Wang Weifang, Zhongguo Jiang. Peach quarantine and identification of Erwinia

1 Scope

This standard specifies the export plant quarantine peach Erwinia quarantine and identification methods. This standard applies to possible quarantine and identification of Erwinia carry peach fruit, seeds and seedlings and other propagation materials.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis SN/T 1809 export plant quarantine rules for seed

3 PRINCIPLE OF THE METHOD

Peach Erwinia (Erwiniapersicina Haoetal.) Belongs to the family Enterobacteriaceae (Enterobacteriaceae), Erwinia (Erwinia). The bacteria can be spread by seeds long distances. The bacteria biological, physiological and biochemical and molecular biological characteristics are The main basis for the development of this standard (see Appendix A).

4 equipment and appliances

4.1 Equipment Biological microscope, clean benches, autoclaves, centrifuges, ultra-low temperature freezer, a conventional refrigerator, incubator, PCR instrument, electrophoresis Equipment and other routine laboratory equipment. 4.2 Appliances Scissors, scalpels, needles vaccination (ring), tweezers, glass rods, dish, alcohol lamp, Erlenmeyer flasks, cylinders, pipettes.

5 Reagents

Unless otherwise specified, all reagents were of analytical grade or biochemical reagents. Test water should comply with GB/T 6682 in a water specifications. See peach media formulations and reagents used Erwinia Appendices B and C. Quarantine and identification of 6 6.1 Field surveys and plant inspection When collecting a field investigation of suspicious symptoms or fruit plants back to the laboratory, cleaned with water, cut out parts of the borderline disease health group Weaving, rinsed once with sterilized water, 75% ethanol disinfection 60s, sterile water 3 times, and then transferred to sterile Petri dish, add a small amount of sterile