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Detection and identification of Erwinia persicina Hao et al.
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SN/T 3965-2014
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Basic data Standard ID | SN/T 3965-2014 (SN/T3965-2014) | Description (Translated English) | Detection and identification of Erwinia persicina Hao et al. | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | B16 | Classification of International Standard | 07.080 | Word Count Estimation | 12,176 | Date of Issue | 4/9/2014 | Date of Implementation | 11/1/2014 | Quoted Standard | GB/T 6682; SN/T 1809 | Regulation (derived from) | State inspection certification (2014) 210 | Issuing agency(ies) | General Administration of Customs | Summary | This standard specifies the export plant quarantine peach Erwinia quarantine and identification methods. This standard applies to possible quarantine and identification of Erwinia carry peach fruit, seeds and seedlings and other propagation materials. |
SN/T 3965-2014: Detection and identification of Erwinia persicina Hao et al.---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Peach quarantine and identification of Erwinia)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Peach quarantine and identification of Erwinia
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Entry Inspection and Quarantine Bureau, Jiangxi Agricultural University.
The main drafters of this standard. Feng Lixia, Cuiru Jiang, what Terry, head Wu Tao, Wang Weifang, Zhongguo Jiang.
Peach quarantine and identification of Erwinia
1 Scope
This standard specifies the export plant quarantine peach Erwinia quarantine and identification methods.
This standard applies to possible quarantine and identification of Erwinia carry peach fruit, seeds and seedlings and other propagation materials.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 1809 export plant quarantine rules for seed
3 PRINCIPLE OF THE METHOD
Peach Erwinia (Erwiniapersicina Haoetal.) Belongs to the family Enterobacteriaceae (Enterobacteriaceae), Erwinia
(Erwinia). The bacteria can be spread by seeds long distances. The bacteria biological, physiological and biochemical and molecular biological characteristics are
The main basis for the development of this standard (see Appendix A).
4 equipment and appliances
4.1 Equipment
Biological microscope, clean benches, autoclaves, centrifuges, ultra-low temperature freezer, a conventional refrigerator, incubator, PCR instrument, electrophoresis
Equipment and other routine laboratory equipment.
4.2 Appliances
Scissors, scalpels, needles vaccination (ring), tweezers, glass rods, dish, alcohol lamp, Erlenmeyer flasks, cylinders, pipettes.
5 Reagents
Unless otherwise specified, all reagents were of analytical grade or biochemical reagents. Test water should comply with GB/T 6682 in a water specifications.
See peach media formulations and reagents used Erwinia Appendices B and C.
Quarantine and identification of 6
6.1 Field surveys and plant inspection
When collecting a field investigation of suspicious symptoms or fruit plants back to the laboratory, cleaned with water, cut out parts of the borderline disease health group
Weaving, rinsed once with sterilized water, 75% ethanol disinfection 60s, sterile water 3 times, and then transferred to sterile Petri dish, add a small amount of sterile
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