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Detection and identification of Tomato severe leaf curl virus
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SN/T 3964-2014
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Basic data | Standard ID | SN/T 3964-2014 (SN/T3964-2014) | | Description (Translated English) | Detection and identification of Tomato severe leaf curl virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 16,172 | | Date of Issue | 4/9/2014 | | Date of Implementation | 11/1/2014 | | Regulation (derived from) | State inspection certification (2014) 210 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method of quarantine and identification of immunology and molecular biology Tomato severe leaf curl virus. This standard applies to identify and quarantine may carry serious tomato leaf curl virus host plants and their products | SN/T 3964-2014: Detection and identification of Tomato severe leaf curl virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Tomato severe leaf curl virus quarantine and identification methods)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Tomato severe leaf curl virus quarantine and identification methods
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Chinese Academy of Inspection and Quarantine, Beijing People's Republic of China Exit Inspection and Quarantine, the People's Republic of China
State and Fujian Entry-Exit Inspection and Quarantine Xiamen People's Republic of China, People's Republic of China Yantai Entry-Exit Inspection
Quarantine Bureau.
The main drafters. Zhang Yongjiang, Deng Cong Liang, Shen Jianguo, Liao Furong, Su Zhi Ping, Lu Jie, water Zhu Fang, Li Mingfu, MATURAL Weimei Sheng.
Tomato severe leaf curl virus quarantine and identification methods
1 Scope
This standard specifies the method of quarantine and identification of immunology and molecular biology Tomato severe leaf curl virus.
This standard applies to identify and quarantine may carry serious tomato leaf curl virus host plants and their products.
2 Tomato severe leaf curl virus basic information
Name. Tomatosevereleafcurlvirus
Abbreviation. ToSLCV
Taxonomic position. The twin virus family (Geminiviridae), bean golden mosaic virus genus (Begomovirus).
Transmission. mainly spread by whiteflies (Bemisiatabaci), it can also be spread by grafting.
Tomato severe leaf curl virus For additional information, see Appendix A.
Principle 3
According to specific reaction Tomato severe leaf curl virus and antibodies between samples were double-antibody sandwich enzyme-linked immunosorbent assay (DAS-
ELISA) detection; conventional PCR or real-time PCR assay based on the specificity of the conserved regions of the viral DNA by electrophoretic bands large
Little or amplification curve of the results of determination.
4 equipment, appliances, and reagents
4.1 Equipment
Reader, ordinary PCR, real-time PCR instrument, multi-purpose electrophoresis, high-speed refrigerated centrifuge, gel imaging system, 4 ℃ ice
Boxes, electronic analytical balance (0.001g), heated water bath, -80 ℃ ultra-low temperature refrigerators, autoclaves and ice machines.
4.2 major appliances
Adjustable pipette (2.5μL, 10μL, 20μL, 100μL, 1000μL), centrifuge tubes, PCR tubes, microtiter plates and mortar and the like.
4.3 Reagents
Unless otherwise stated, all test reagents were analytical grade or biochemical reagents.
Immunosorbent assay enzyme-linked double antibody sandwich (DAS-ELISA) reagent Appendix B, conventional PCR detection reagent in Appendix C.
5 quarantine and identification methods
5.1 Preparation of the test sample
5.1.1 seedlings. symptoms (such as plant height, leaf malformation, curled, wrinkled, etc.) seedlings individually numbered, with each plant collection of symptoms blade 5g
For subsequent detection; asymptomatic plants are divided into 10 per group and number of leaves at the top of 5g mixed collection for subsequent detection.
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