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Detection of Peach rosette mosaic virus
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SN/T 3963-2014
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Basic data | Standard ID | SN/T 3963-2014 (SN/T3963-2014) | | Description (Translated English) | Detection of Peach rosette mosaic virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 16,113 | | Date of Issue | 4/9/2014 | | Date of Implementation | 11/1/2014 | | Regulation (derived from) | State inspection certification (2014) 210 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method for inspection and identification peach rosette mosaic virus. This standard applies to quarantine and identification of peach, grapes and other plant material peach rosette mosaic virus. | SN/T 3963-2014: Detection of Peach rosette mosaic virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Peach rosette mosaic virus detection method)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Peach rosette mosaic virus detection methods
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Zhejiang Exit Inspection and Quarantine, the People's Republic of China
State and Tianjin Entry-Exit Inspection and Quarantine, Ningbo People's Republic of China.
The main drafters of this standard. ETHNIC SOCIETY OF Guo Jingze, Wen Weigang, Liming Fu, Zhu Shuifang.
Peach rosette mosaic virus detection methods
1 Scope
This standard specifies the method for inspection and identification peach rosette mosaic virus.
This standard applies to quarantine and identification of peach, grapes and other plant material peach rosette mosaic virus.
Principle 2
Peach rosette mosaic virus Scientific name. Peachrosettemosaicvirus, Abbreviation. PRMV. Its specific groups of the virus coat protein
Specificity of the genomic nucleic acid sequence is the main basis of this standard-setting. Related Information See Appendix A.
3 equipment, appliances, and reagents
3.1 Equipment
ELISA detector, real-time PCR instrument, low temperature refrigerators, incubators, autoclaves, electronic analytical balance, high-speed desktop centrifuged
Machine, constant temperature water bath, ice-making machines.
3.2 Appliances
Micro adjustable pipette (range, respectively 2.5μL, 10μL, 100μL, 200μL and 1mL) and sterilized tip, 1.5mL centrifugation
Tube, mortar, ELISA plate, 0.2mLPCR tubes, 96-well PCR reaction plates and lids.
3.3 Reagents
Reagents, buffers, primers and probe sequences, etc. See Appendix B and Appendix C.
4 Detection and Identification
4.1 Serology (double antibody sandwich enzyme-linked immune assay)
4.1.1 Coating antibody
The PRMV antibody with the coating buffer was diluted to working concentration, enzyme-linked plate, each hole by adding 100μL. The ELISA plate stamped
(Or sealed with plastic wrap) into the humidor, placed in 37 ℃ incubator incubated for 2h (or 4 ℃ refrigerator overnight). After each well was added
300μLPBST washed three times, each time 3min.
4.1.2 Sample Preparation
Plant leaves to be tested was weighed 0.2g, was added 2mL sample extraction buffer, followed by sufficient grinding, into a 1.5mL centrifuge tube,
5000r/min centrifugal 5min, the supernatant 1.10 dilution of the sample is a crude extract.
4.1.3 sample
The sample was added to the crude extract of enzyme-linked antibody-coated plates, each well 100μL. Konga buffer as control, at the same time set
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