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SN/T 3954-2014 English PDF

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SN/T 3954-2014: Detection of nipah virus at frontier port by RT-PCR and real time fluorescence RT-PCR
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PDF similar to SN/T 3954-2014


Standard similar to SN/T 3954-2014

GBZ 57   GB/T 31989   SN/T 3953   SN/T 3955.1   SN/T 3952   

Basic data

Standard ID SN/T 3954-2014 (SN/T3954-2014)
Description (Translated English) Detection of nipah virus at frontier port by RT-PCR and real time fluorescence RT-PCR
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C62
Classification of International Standard 13.020
Word Count Estimation 12,121
Date of Issue 4/9/2014
Date of Implementation 11/1/2014
Quoted Standard GB 19489; SN/T 1193; WS/T 230
Regulation (derived from) State inspection certification (2014) 210
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the object border crossings Nipah virus by RT-PCR and real-time RT-PCR method testing, testing methods and results reporting. This standard applies to border crossings Nipah virus in laboratory tests.

SN/T 3954-2014: Detection of nipah virus at frontier port by RT-PCR and real time fluorescence RT-PCR


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(Border crossings Nipah virus RT-PCR and real-time fluorescence RT-PCR detection method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Border crossings Nipah virus RT-PCR and real-time fluorescence RT-PCR detection method Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, Shenzhen Academy of Metrology and Quality Inspection, Chinese people Republic of Sichuan Exit Inspection and Quarantine. The main drafters of this standard. Mo Qiu-hua, Li Weigang, Tan Hua, Shi Yongmei Hu Kexin, blue whole school, Lin Jican, Ze, Zhao Junhua, Chen Xiao Xiao. Border crossings Nipah virus RT-PCR and real-time fluorescence RT-PCR detection method

1 Scope

This standard specifies the object border crossings Nipah virus by RT-PCR and real-time RT-PCR method testing, testing methods and Report the results. This standard applies to border crossings Nipah virus in laboratory tests.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 19489 General requirements for laboratory biosafety SN/T 1193 genetic testing laboratory technical requirements Applications WS/T 230 clinical diagnostic polymerase chain reaction (PCR) technology Can infect humans highly pathogenic microorganisms (viruses) or sample transport regulations (formerly People's Republic of China Ministry of Health) Human infection of pathogenic microorganisms (viruses) Collection agency management approach (the former People's Republic of China Ministry of Health) Human infection of pathogenic microorganisms Directory (formerly People's Republic of China Ministry of Health, 2006)

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Nipah virus Nipahvirus; NiV Nipah virus Nipahvirus; NiV Belongs to the paramyxovirus family (Paramyxoviridae) 亨德拉尼帕 virus genus (Henipavirus), single-stranded negative-strand RNA viruses, may be cited From a novel potent zoonotic infectious diseases --- Nipah virus disease (Nipahvirusdisease, NVD), a serious threat to humans and pigs, Healthy horses and other animals. Nipah virus infection of human incubation period after 1 week to 3 weeks, it was possible successor in the crowd, the clinical manifestations of human The main severe rapidly progressive encephalitis, brainstem dysfunction (manifested as high blood pressure, tachycardia), the mortality rate as high as 40% to 70%. Do not Severity with onset vary, most nerve symptoms, respiratory symptoms may be characterized by symptoms are neck and abdominal muscles Spasms, while there body temperature, headache, drowsiness, vomiting, cough, confusion, severe coma and death. 3.2 Reverse transcriptase polymerase chain reaction reversetranscriptionpolymerasechainreaction; RT-PCR The reverse transcription polymerase chain RNA (RT) and cDNA amplification (PCR) a combination of techniques. The standard one-step inverse Transcriptase polymerase chain reaction (RT-PCR). In the one-step RT-PCR, first RNA as a template, use the downstream specific primers, Transcription to synthesize the complementary DNA (cDNA) in the RNA-dependent DNA polymerase (reverse transcriptase) action, this step is called "reverse"; Sui , And then to the cDNA as a template, the upstream and downstream primers, under the action of DNA polymerase for PCR amplification cycles; most An end to a particular region of the RNA molecule (fragment) is amplified up to several hundred times.

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