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SN/T 3952-2014 English PDF

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SN/T 3952-2014: Detection of influenza A virus at frontier port - Dot-ELISA
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PDF similar to SN/T 3952-2014


Standard similar to SN/T 3952-2014

GBZ 57   GB/T 31989   SN/T 3954   SN/T 3955.1   SN/T 3953   

Basic data

Standard ID SN/T 3952-2014 (SN/T3952-2014)
Description (Translated English) Detection of influenza A virus at frontier port - Dot-ELISA
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C62
Classification of International Standard 13.020
Word Count Estimation 8,848
Date of Issue 4/9/2014
Date of Implementation 11/1/2014
Quoted Standard GB 19489; SN/T 2752.1
Regulation (derived from) State inspection certification (2014) 210
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the acquisition and processing, enzyme-linked immunosorbent assay for the detection step terms and definitions influenza A virus, bio-security requirements, samples determination results, test reports and positive disposition. This

SN/T 3952-2014: Detection of influenza A virus at frontier port - Dot-ELISA

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Border crossings influenza virus detection method Dot-ELISA method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Border crossings influenza virus detection method Dot-ELISA method Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine, Xiamen University School of Public Health. The main drafters of this standard. Yangkun Yu, Chen Hui, Wu Bingbing, Zhang Yi, Li Shu Gan, Chen Fan, Chen Yixin, Lin Qing, Yang Jian. Border crossings influenza virus detection method Dot-ELISA method

1 Scope

This standard specifies the terms and definitions acquisition and processing influenza A virus, bio-security requirements, sample, enzyme-linked immunosorbent percolation Detecting step method, the result is determined to detect and report a positive result for disposal. This standard applies to people infected with influenza virus detected in border crossings.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 19489 General requirements for laboratory biosafety Self-protection norms SN/T 2752.1 quarantine personnel

3 Terms and Definitions

The following terms and definitions apply to this document. 3.1 Influenza virus influenzaAvirus Influenza viruses belong to Orthomyxoviridae (Orthomyxoviridae), it is a spherical or rod-shaped, enveloped single-stranded negative RNA viruses. Influenza A virus can infect birds, and mammals such as cats, depending on the surface glycoproteins hemagglutinin (HA) and nerve ammonia Enzyme acid (NA) antigens of different influenza viruses can be divided into 16 H and 9 N subtypes. Influenza A virus can cause acute respiratory Respiratory tract diseases, the disease primarily through droplets in the air, human contact, contact with contaminated items spread. The typical clinical symptoms Like for the rapid fever, aching muscles and fatigue. 3.2 Nucleoprotein nucleocapsid; NP By influenza virus genome segments encoding 5 containing 498 amino acids, a non-phosphorylated monomer glycosylated proteins, and 3 Kinds of RNA polymerase protein RNA together form the body (RNPs), RNPs and RNA polymerase together constitute the core of the virus. NP most conservative in the eight influenza virus genes having type-specific, the influenza virus is divided into A, B, C three kinds of type important basis. 3.3 ELISA filtration assay dot-enzymelinkedimmunosorbnentassay; dot-ELISA Influenza ELISA method using enzyme-linked immunosorbent percolation principle of double-antibody sandwich method and immune infiltration technique for detecting samples Influenza virus nucleoprotein NP antigen. In nitrocellulose membrane coated with anti-NP antigen monoclonal antibodies, the test sample by lysis buffer After treatment, the nitrocellulose capture monoclonal antibodies, formation of an antigen - antibody complex, and then adding the enzyme-labeled antibody anti-NP antigen, shaped Into antibody - antigen - enzyme-antibody complex, and finally through enzymatic chromogenic substrate determination result. 4 bio-safety requirements Personal protective field sampling personnel should comply with SN/T 2752.1 requirements, structure and laboratory facilities, safe working practices, security settings

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