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SN/T 3937-2014: Determination of choline phosphate in drinks for export - LC - MS/MS method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 3937-2014 | English | 319 |
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Determination of choline phosphate in drinks for export - LC - MS/MS method
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SN/T 3937-2014
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Basic data | Standard ID | SN/T 3937-2014 (SN/T3937-2014) | | Description (Translated English) | Determination of choline phosphate in drinks for export - LC - MS/MS method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X50 | | Classification of International Standard | 67.160 | | Word Count Estimation | 12,13 | | Date of Issue | 4/9/2014 | | Date of Implementation | 11/1/2014 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | State inspection certification (2014) 210 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the phosphorylcholine beverage liquid chromatography - mass spectrometry/mass spectrometry. This standard applies to fruit drinks, sports drinks, wine, beer and sake in phosphorylcholine detection. | SN/T 3937-2014: Determination of choline phosphate in drinks for export - LC - MS/MS method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Determination of export beverage phosphorylcholine liquid chromatography - mass spectrometry/mass spectrometry)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of export beverage phosphorylcholine
Liquid chromatography - mass spectrometry/mass spectrometry
LC-MS/MSmethod
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Inner Mongolia, People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Pan Guoqing, Zheng Book Fair, Li Gang, Baiguo Tao, Chun - Yan Zhang, Lu Hua Wei, Guo Tie Cheng.
Determination of export beverage phosphorylcholine
Liquid chromatography - mass spectrometry/mass spectrometry
1 Scope
This standard specifies the phosphorylcholine beverage liquid chromatography - mass spectrometry/mass spectrometry.
This standard applies to fruit drinks, sports drinks, wine, beer and sake in phosphorylcholine detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Method summary
The sample was diluted with water extraction, ion-exchange solid-phase extraction (SPE) (alcoholic beverages using HLB solid phase extraction column pre-purification), with liquid
Chromatography - mass spectrometry/mass spectrometer, external standard.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.1 Methanol. chromatographically pure.
4.2 formic acid. Chromatographic pure.
4.3 acetonitrile. chromatography.
4.4 ammonium acetate. AR.
4.5 20mmol/L ammonium acetate solution. 1.54g ammonium acetate dissolved in 1000mL water.
4.6 10% methanol solution of formic acid. 10.0mL acid was dissolved in methanol, treated with methanol to 100mL.
4.7 phosphorylcholine chloride standard (CAS. 107-73-3, C5H15ClNO4P). 99%.
4.8 phosphorylcholine standard stock solution. Weigh 0.0120g choline chloride phosphate standard (4.7), dissolved in water volume to 100.0mL, dissolved
Concentration of 100μg/mL, 1 ℃ ~ 4 ℃ cold storage, valid for six months.
4.9 phosphorylcholine standard solution. acetonitrile - water (11 volume) was diluted stock solution, formulated to concentrations of 5ng/mL,
10ng/mL, 50ng/mL, 100ng/mL, 200ng/mL, 500ng/mL standard working solution, now with the existing service.
4.10 SPE. HLB1) solid phase extraction column, 200mg, rinsed before use with water and methanol each 5mL. SAX SPE,
500mg, rinsed with methanol before use and 20mmol/L ammonium acetate solution each 5mL.
1) non-commercial Disclaimer. This test is used WatersHLB SPE accomplished listed here provide a reference test with SPE models only,
It does not involve commercial purposes, to encourage users to try the standard solid phase extraction column using a different manufacturer or model.
4.11 0.22μm aqueous membrane.
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