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SN/T 1607-2017: Total bacterial count，coliforms，fecal coliforms and Escherichia coli counts in drinks for export. Hydrophobic grid membrane filtration(HGMF) method
Status: Valid SN/T 1607: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1607-2017 | English | 189 |
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Total bacterial count，coliforms，fecal coliforms and Escherichia coli counts in drinks for export. Hydrophobic grid membrane filtration(HGMF) method
| Valid |
SN/T 1607-2017
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| SN/T 1607-2005 | English | 279 |
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Total bacterial count, coliform, fecal coliform and Escherichia coli counts in drink for import and export.Hydrophobic grid membrane filtration method
| Obsolete |
SN/T 1607-2005
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PDF similar to SN/T 1607-2017
Basic data | Standard ID | SN/T 1607-2017 (SN/T1607-2017) | | Description (Translated English) | Total bacterial count��coliforms��fecal coliforms and Escherichia coli counts in drinks for export. Hydrophobic grid membrane filtration(HGMF) method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X50 | | Classification of International Standard | 67.160 | | Word Count Estimation | 8,862 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Older Standard (superseded by this standard) | SN/T 1607-2005 | | Regulation (derived from) | National Quality Inspection (2017) No. 337 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method of counting the total number of colonies, coliforms, fecal coliforms and E. coli in the exported beverages by the hydrophobic grid filter method. This standard applies to the total number of colonies, coliforms, fecal coliforms and E. coli in exported packaging beverages. | SN/T 1607-2005: Total bacterial count, coliform, fecal coliform and Escherichia coli counts in drink for import and export.Hydrophobic grid membrane filtration method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Total bacterial count, coliform, fecal coliform and Escherichia coli counts in drink for import and export.Hydrophobic grid membrane filtration method
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Total number of colonies in the import and export beverages, coliforms,
Fecal coliform, E. coli counting method
Hydrophobic grid filter
Released on.2005-08-18
2006-02-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard is mainly drafted by. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Guan Yu.
This standard is the first industry standard for entry-exit inspection and quarantine.
Total number of colonies in the import and export beverages, coliforms,
Fecal coliform, E. coli counting method
Hydrophobic grid filter
1 Scope
This standard specifies the total number of colonies, coliforms, fecal coliforms and E. coli in the import and export beverages.
Membrane method.
This standard is applicable to the determination of drinking and drinking natural mineral water, bottle (barrel) drinking pure water, tea beverage and carbonated beverage.
2 Method summary
When a certain amount of sample liquid passes through the filter membrane, the bacteria are trapped in the square of the filter membrane, and after the filter membrane is cultured on the medium, the bacteria are counted.
The number of colonies can be measured to determine the total number of colonies; if the filter is placed on selective medium, the positive colonies will be colored in a specific color, counting
The number of positive colonies in these specific colors can be used to determine the number of positive colonies in the sample.
3 media and reagents
3.1 Pancreatized soybean solid green agar see Appendix A.
3.2 MFC agar see Appendix A.
3.3 Pancreatized soybean magnesium sulfate agar see Appendix A.
3.4 Tryptone bile agar see Appendix A.
3.5 See Appendix A for hydrazine reagents.
3.6 sterile saline.
4 Equipment and materials
4.1 Hydrophobic grid membrane. The pore size is 0.45 μm.
4.2 Filter.
4.3 Incubator. 36 ° C ± 1 ° C, 44.5 ° C ± 0.5 ° C.
4.4 Vacuum pump.
4.5 Filter bottle.
4.6 Sterile forceps.
4.7 Pipette. 1 mL pipette with 0.1 mL scale, 10 mL pipette with 1 mL scale.
4.8 Autoclave.
4.9 Petri dish. 90mm in diameter.
4.10 Filter paper.
5 sample preparation
5.1 Samples with low bacterial content can be directly filtered using a sterile pipette with 50 mL.
5.2 Based on an estimate of the contamination of the sample, the sample is made into a series of 10-fold increasing sample dilutions using sterile saline. preparation
The entire sample should not be allowed to exceed 15 min and then filtered with 50 mL of sample dilution.
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