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Determination of 6 arsenic speciations in export food - High performance liquid chromatography-inductively coupled plasma mass spectrometry
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SN/T 3933-2014
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Basic data | Standard ID | SN/T 3933-2014 (SN/T3933-2014) | | Description (Translated English) | Determination of 6 arsenic speciations in export food - High performance liquid chromatography-inductively coupled plasma mass spectrometry | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 16,137 | | Date of Issue | 4/9/2014 | | Date of Implementation | 11/1/2014 | | Quoted Standard | GB/T 6682-2008 | | Regulation (derived from) | State inspection certification (2014) 210 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the export of food Central Asia arsenate arsenate, arsenic choline, betaine arsenic, monomethyl and dimethyl arsenic arsenic forms arsenic content of six kinds of high performance liquid chromatography - inductively coupled plasma |
SN/T 3933-2014: Determination of 6 arsenic speciations in export food - High performance liquid chromatography-inductively coupled plasma mass spectrometry ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Six food exports arsenic speciation method for measuring performance liquid chromatography - inductively coupled plasma mass spectrometry)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of six food exports Arsenic Speciation
High Performance Liquid Chromatography - Inductively Coupled Plasma Mass Spectrometry
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shandong CIQ.
The main drafters of this standard. Yang Lijun, COLLECE Hu Qiaoru, Song Xiaohua, Cuifeng Jie, Liu Yumin, Lu Guoqiang.
Determination of six food exports Arsenic Speciation
High Performance Liquid Chromatography - Inductively Coupled Plasma Mass Spectrometry
1 Scope
This standard specifies the export of food Central Asia arsenate arsenate, arsenic choline, betaine arsenic, monomethyl and dimethyl arsenic arsenic-containing six kinds of arsenic speciation
High Performance Liquid Chromatography - inductively coupled plasma mass spectrometry.
This standard applies to meat, eggs, dairy, fruits, vegetables, fruit juice, wine, sugar, algae, fish, food and mineral water in Central Asia arsenate class
, Arsenate, arsenic and arsenic choline betaine, a quantitative methyl arsenic, arsenic and dimethyl arsenic six kinds of shape measurement.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682-2008 Analysis
3 Method summary
Six kinds of arsenic speciation in a sample by artificial gastric juice or methanol ultrasonic extraction solution, after centrifugation, membrane purification, high performance liquid chromatography - inductively
Coupled Plasma Mass Spectrometry, external standard.
4 Reagents and materials
Unless otherwise noted, all reagents were of analytical grade water as a water GB/T 6682-2008 requirements.
4.1 Methanol. chromatographically pure.
4.2 Concentrated hydrochloric acid. excellent pure content of 36.0% - 38.0%.
Ammonia 4.3. Content (NH3) 25% ~ 28%.
4.4 Pepsin. each containing 149 mg of active units.
4.5 ammonium carbonate. C2H11N3O5, commercial, pure class distinctions, content (NH3 dollars) 40.0%.
4.6 with dilute hydrochloric acid. Take 324mL of concentrated hydrochloric acid (4.2), diluted with water to 1000mL, and mix.
4.7 10% methanol solution. Take 100mL of methanol (4.1), diluted with water and dilute to 1000mL, and mix.
4.8 artificial gastric juice. Pipette 16.4mL dilute hydrochloric acid (4.6), add water to about 800mL and pepsin 10g, mix, diluted with water to 1000mL,
Using now.
4.9 50mmol/L ammonium carbonate solution. Weigh 7.86g of ammonium carbonate (4.5), dissolved in water and diluted to 1000mL, adjusted with ammonia (4.3)
pH to 9.5, and mix, using now.
4.10 Standard substances. arsenite [Arseniousacid, As (Ⅲ)], AsO3-3; arsenate [Arsenicacid, As (Ⅴ)], AsO3-4;
Arsenocholine (Arsenocholine, AsC), C5H14AsBrO; arsenic betaine (Arsenobetaine, AsB), C5H11AsO2; monomethyl arsenic
(Monomethylarsonicacid, MMA), CH3AsO2-3; dimethyl arsenic (Dimethylarsinicacid, DMA), C2H7AsO2.
4.11 standard stock solution (5.0μg/mL). six kinds of arsenic were taken form the standard substance (4.10), with the amount of dissolved water, equipped with standard stock solution
Solution, the standard concentration of the stock solution of each form of arsenic 5.0μg/mL. At 0 ℃ ~ 4 ℃ dark preservation, valid for three months.
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