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SN/T 3932-2014 English PDF

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SN/T 3932-2014: Rapid detection of Bacillus Cereus for export foods - Real-time PCR method
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Basic data

Standard ID SN/T 3932-2014 (SN/T3932-2014)
Description (Translated English) Rapid detection of Bacillus Cereus for export foods - Real-time PCR method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 8,897
Date of Issue 4/9/2014
Date of Implementation 11/1/2014
Quoted Standard GB/T 4789.14
Regulation (derived from) State inspection certification (2014) 210
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the real-time PCR detection methods in food for export of Bacillus cereus. This standard applies to the rapid detection of food for export of Bacillus cereus.

SN/T 3932-2014: Rapid detection of Bacillus Cereus for export foods - Real-time PCR method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Bacillus cereus rapid detection method in real-time quantitative PCR and food exports) People's Republic of China Entry-Exit Inspection and Quarantine Standards Food exports Bacillus cereus rapid detection method Real-time quantitative PCR Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

In accordance with this standard GB/T 1.1-2009 given rule drafting. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai Jiaotong University, the People's Republic of China Hubei Entry Inspection and Quarantine Bureau, People's Republic of China Shandong CIQ. Drafters of this standard. Yang Jie Lin, Shi Chunlei, Chen Hung Chao, Zengxian Dong, willow, Jia Juntao, He Yuping, Panliang Wen, Zhang Jilun, Jiang Yinghui. Food exports Bacillus cereus rapid detection method Real-time quantitative PCR

1 Scope

This standard specifies the real-time PCR detection methods in food for export of Bacillus cereus. This standard applies to the rapid detection of food for export of Bacillus cereus.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 4789.14 Microbiological examination of food hygiene Bacillus cereus test

3 Determination

3.1 Method summary This standard uses the lock nucleotides (LNA) probes specifically detected Bacillus cereus. The probe utilizes a single nucleotide polymorphism (SN P, SingleNucleotidePolymorphism) principle, the use of Bacillus cereus from other Bacillus species specific sites Testing. 3.2 Reagents and materials Unless otherwise specified, reagents were analytical grade or biochemical reagents, water sterile double distilled water. 3.2.1 primer. 5'-CCTTCTTCAAGTTCAAATCTCG-3 ' 5'-GTYGTAATGACAGGTGATGGA-3 ' 3.2.2 Lock nucleotides (LNA) probes. FAM-5'-FAM-TGTAAT * GG * TTGTT * CG * CAA-BHQ1-3 ' Note. * indicates that the site is locked nucleotide sites. 3.2.3 TaqDNA polymerase. 3.2.4 dNTP. dATP, dTTP, dCTP, dGTP. 3.2.5 DNA Extraction Reagents. 0.1% Chelex100 solution. 3.2.6 10 × PCR buffer.200mmol/LTris-HCl (pH8.4), 200mmol/L KCl, 15mmol/L magnesium chloride. 3.2.7 LB medium. Weigh casein peptone 10g, yeast extract 5g, NaCl10g, dissolved in 1L of water, adjusting the pH to 7.0, high Autoclaving. 3.2.8 Quality control strains of Bacillus cereus. ATCC14579 or equivalent strains. 3.3 instruments and equipment 3.3.1 fluorescent PCR instrument. 3.3.2 Centrifuge.20000r/min.

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