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SN/T 3750-2013: Detection and identification of Botryosphaeria stevensii Shoemaker
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Detection and identification of Botryosphaeria stevensii Shoemaker
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SN/T 3750-2013
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Basic data | Standard ID | SN/T 3750-2013 (SN/T3750-2013) | | Description (Translated English) | Detection and identification of Botryosphaeria stevensii Shoemaker | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 12,128 | | Quoted Standard | SN/T 2122 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the phytosanitary single shell color in apple canker quarantine across the spore identification method. This standard applies to apples across the shell color single spore identification canker quarantine all host plants of wood, p | SN/T 3750-2013: Detection and identification of Botryosphaeria stevensii Shoemaker---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Botryosphaeria stevensii Shoemaker
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Apple shell color single compartment spore canker quarantine and identification methods
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine.
The main drafters of this standard. Wang Weifang, Zhang Qiue, Huang Ying, Yan Jin, gorgeous.
Apple shell color single compartment spore canker quarantine and identification methods
1 Scope
This standard specifies the phytosanitary Apple shell color in a single compartment Alternaria canker quarantine authentication methods.
This standard applies to apples shell color single compartment spore identification canker quarantine all host plants of logs, branches, nursery stock and fruit.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 2122 entry and exit quarantine of plants and plant products sampled
Principle 3
According to Apple shell color single compartment spore canker infestation symptoms, colony characteristics, asexual bacteria type and type of sexual morphology of the pathogen detection
Phytophthora identification.
4 Reagents and Equipment
4.1 Media and Reagents
Potato dextrose agar (PDA. the 200g per 1000mLPDA peeled potato, 20g glucose, 18g agar with
From the system), oatmeal agar (OA. oatmeal 30g per 1000mLOA made, 20g agar preparation), 100% lactic acid, 1%
Sodium hypochlorite (NaOCl), 70% ethanol and sterile water.
4.2 instruments and equipment
Stereo microscope, biological microscope (with oil immersion and micrometer), the general balance (inductance 0.1g), clean benches, biological incubator, high pressure
Sterilizer, slides, cover slips, hole punch (diameter 4mm).
5 quarantine and identification
5.1 Symptom Check
Check the extraction of logs, wood products, surface shoots and seedlings bark if ulcers, if there are dead branches symptoms, check whether fruit
Rot symptoms (symptom description and pictures see A.3, Figure B.1).
Press SN/T 2122 sampling, there will be samples of suspicious symptoms back to the laboratory for further examination.
5.2 Laboratory Accreditation
5.2.1 Morphology of bacteria in the sample
Fruiting body collected after the cross-sections from the bark tissue sections were transferred to drops of sterile water slides, dried at room temperature plus
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