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SN/T 3577-2013 English PDF

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SN/T 3577-2013: Detection of genetically modified components. Method for cotton using PCR-DHPLC
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PDF similar to SN/T 3577-2013


Standard similar to SN/T 3577-2013

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Basic data

Standard ID SN/T 3577-2013 (SN/T3577-2013)
Description (Translated English) Detection of genetically modified components. Method for cotton using PCR-DHPLC
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B32
Classification of International Standard 65.020
Word Count Estimation 8,893
Quoted Standard GB/T 19495.7; GB/T 6682; SN/T 1193
Regulation (derived from) AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall)
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the entry and exit of genetically modified cotton and PCR-DHPLC detection lines. The standard detection methods applied to transgenic cotton lines MON531, MON1445, MON1698, MON15985 and strains of genetically modified tests.

SN/T 3577-2013: Detection of genetically modified components. Method for cotton using PCR-DHPLC

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of genetically modified components. Method for cotton using PCR-DHPLC People's Republic of China Entry-Exit Inspection and Quarantine Standards GMO detection Cotton PCR-DHPLC Detection Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, China Inspection Quarantine Science Research Institute, Shenzhen Bo Rui Xiang Hui Biotechnology Co., Ltd., National Center for Nanoscience. The main drafters. Zhang Guiming, water Zhu Fang, only to Yu, Cheng Yinghui, apricot Ling, Wang Zhaohui, PLAIN, Huang Xin, Ouyang Hui, Wang Ying, Zhong Jianzhong. GMO detection Cotton PCR-DHPLC Detection

1 Scope

This standard specifies the entry and exit of genetically modified cotton and PCR-DHPLC detection lines. The standard method is suitable for the detection of genetically modified cotton line MON531, MON1445, MON1698, MON15985 in transgenic Due to component and line detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.7 GMO detection methods of sampling and sample preparation SN/T 1193 genetic testing laboratory technical requirements Principle 3 Denaturing high-performance liquid chromatography (denaturinghigh-performanceliquidchromatography, DHPLC) is a simple Single, rapid, nucleic acid analysis method of non-gel at 50 ℃ condition of sample analysis, the number of base pairs in the sample determines the order of elution peak, when over Column acetonitrile concentration increases, the nucleic acid fragment will be from small to large order was eluted according to the relative molecular mass. This standard uses the multiplex PCR Methods for transgenic cotton containing genetically modified to amplify the same time, the amplified products were analyzed by DHPLC. By DHPLC The elution peak was determined by comparison with molecular weight marker size, it determines whether or not with a foreign gene and determine the composition of genetically modified cotton strain. 4 equipment, utensils and reagents 4.1 Equipment and Utensils Tissue grinder, PCR instrument, DHPLC meter, water bath, electronic balance (precision. 1/1000 above), ordinary centrifuge, freezing high Speed centrifuges, refrigerators, cold refrigerator, ice maker, oven temperature, pH meter, pipettes (0.1μL, 0.5μL, 2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), UV-visible spectrophotometer, water machines, autoclaves, PCR tubes (0.2mL), centrifuge tubes (1.5mL, 2.0mL), Tip head (0.1μL ~ 10μL, 5μL ~ 200μL, 100μL ~ 500μL). 4.2 Reagents CTAB extract, Tris saturated phenol, chloroform, isoamyl alcohol, isopropyl alcohol, 70% ethanol, RNA enzyme (10mg/mL), multiplex PCR The reaction buffer solution (MultiplexPCRMix), TaqDNA polymerase, DHPLC Buffer A. TEAA (0.1mol/L, pH7.0), B Nitrile (0.025%), DHPLC Buffer B. TEAA (0.1mol/L, pH7.0), acetonitrile (25%), DHPLC buffer D. acetonitrile (75%, pH7.0), molecular weight marker (marker).

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