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SN/T 3581-2013 English PDF

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SN/T 3581-2013: Detection and identification of Monilinia fructicola(Winter)Honey with real-time fluorescence PCR
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
SN/T 3581-2013	English	189	Add to Cart	3 days [Need to translate]	Detection and identification of Monilinia fructicola(Winter)Honey with real-time fluorescence PCR	Valid	SN/T 3581-2013

Standard similar to SN/T 3581-2013

SN/T 5268

Basic data

Standard ID	SN/T 3581-2013 (SN/T3581-2013)
Description (Translated English)	Detection and identification of Monilinia fructicola(Winter)Honey with real-time fluorescence PCR
Sector / Industry	Commodity Inspection Standard (Recommended)
Word Count Estimation	7,728
Quoted Standard	GB/T 6682; SN/T 1193; SN/T 1871
Regulation (derived from)	?AQSIQ About publishing 2013 First Batch 179-items Entry-Exit Inspection and Quarantine industry standard Announcement; Industry-Standard-Filing Announcement 2013 No. 9 (Total No. 165)
Issuing agency(ies)	General Administration of Customs
Summary	This standard specifies the US-Australia -type stone brown rot [Monilinia fructicola (Winter) Honey] application TaqMaNmGB probe of real-time PCR detection methods. This standard applies to the United States and Australia -based brown rot of stone fruit h

SN/T 3581-2013: Detection and identification of Monilinia fructicola(Winter)Honey with real-time fluorescence PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Monilinia fructicola (Winter) Honey with real-time fluorescence PCR People's Republic of China Entry-Exit Inspection and Quarantine Standards United States and Australia type stone fructicola real-time PCR Detection method DetectionandidentificationofMoniliniafructicola (Winter) Honey Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Structure does not bear the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute. The main drafters of this standard. Cheng Yinghui, Wang Ying, Zhang Guiming, Wang Ying. United States and Australia type stone fructicola real-time PCR Detection method

1 Scope

This standard specifies the United States and Australia type stone fructicola [Moniliniafructicola (Winter) Honey] Application TaqManMGB Probe detection method of real-time PCR. This standard applies to the United States and Macao type stone fructicola hosts include stone fruits, and Chaenomeles, Crataegus, Eriobotrya preach with US The molecular detection of pathogen type O drupe fructicola on the host.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis SN/T 1193 genetic testing laboratory technical requirements SN/T 1871 American-Australian-type drupe fructicola quarantine and identification methods Principle 3 3.1 Status classification and morphology of bacteria According to SN/T 1871. 3.2 Identification of principle According to the United States and Macao type stone fructicola its approximate species including stone fruit brown rot bacteria and European pome fruit brown rot bacteria interval transcribed region of the gene Different design specific primers and probes, application-related instruments, including the real-time PCR instrument, such as the United States Ao stone type brown rot germs Real-time PCR for identification. 4 major appliances, instruments and reagents 4.1 The main instruments and appliances Oven, Autoclave, Balance (a sense of volume 1/100,1/10000), mortar, shaker, water bath, ice machines, water meter, Vortex, Tabletop centrifuge, nucleic acid protein analyzer, high-speed refrigerated centrifuge, vacuum drained instrument, refrigerator, clean benches, real-time PCR instrument, trace Pipettes (0.5μL, 2μL, 10μL, 20μL, 100μL, 200μL, 1000μL), centrifuge tubes (0.2mL, 1.5mL, 2.0mL), Tip head (0.1μL ~ 10μL, 5μL ~ 200μL, 100μL ~ 1000μL), test tubes. 4.2 Reagents CTAB extract, Tris saturated phenol, chloroform, isoamyl alcohol, isopropanol, ethanol, RNA enzymes, liquid nitrogen, real-time PCR reactions Buffer.

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