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SN/T 3567.9-2017 English PDF

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SN/T 3567.9-2017: Detection method of crossing priming isothermal amplification - Part 9: Yersinia pestis
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PDF similar to SN/T 3567.9-2017


Standard similar to SN/T 3567.9-2017

GBZ 57   GB/T 31989   SN/T 3567.11   SN/T 3567.7   SN/T 3567.10   

Basic data

Standard ID SN/T 3567.9-2017 (SN/T3567.9-2017)
Description (Translated English) Detection method of crossing priming isothermal amplification - Part 9: Yersinia pestis
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C62
Classification of International Standard 13.020
Word Count Estimation 9,937
Date of Issue 2017-07-21
Date of Implementation 2018-03-01
Quoted Standard GB/T 6682; GB 19489; SN/T 1280-2014; WS 279-2008
Regulation (derived from) State-Quality-Inspection-Accreditation (2017) 337
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the object, test procedure and test results of the detection of Yersinia pestis cross-primer constant temperature amplification method at the border port. This standard is applicable to the screening test of Yersinia pestis and the laboratory testing of Yersinia pestis in entry and exit personnel at border ports.

SN/T 3567.9-2017: Detection method of crossing priming isothermal amplification - Part 9: Yersinia pestis


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Detection method of crossing priming isothermal amplification - Part 9. Yersinia pestis People's Republic of China entry and exit inspection and quarantine industry standards Cross primer constant temperature amplification detection method Part 9. Yersinia pestis Part 9. Yersiniapestis Released on.2017-07-21 2018-03-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

SN/T 3567 "Cross Primer Constant Temperature Amplification Detection Method" is divided into 11 parts. --- Part 1. General technical procedures; --- Part 2. Vibrio cholerae; --- Part 3. E. coli O157.H7; --- Part 4. Yellow fever virus; --- Part 5. Salmonella; --- Part 6. Mycobacterium tuberculosis; --- Part 7. Enterovirus 71; --- Part 8. Plasmodium; --- Part 9. Yersinia pestis; ---Part 10. Bacillus anthracis; --- Part 11. Shigella. This part is the ninth part of SN/T 3567. This part is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This part is proposed and managed by the National Certification and Accreditation Administration. This section drafted by. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Hebei Entry and Exit Inspection and Quarantine Bureau, Hangzhou Yousida Biotechnology Co., Ltd. The main drafters of this section. Wang Xin, Guan Yu, Lan Jing, Li Haochen, Qi Jun, Hu Lin, especially Min. Cross primer constant temperature amplification detection method Part 9. Yersinia pestis

1 Scope

This part of SN/T 3567 specifies the objects, testing procedures and detection procedures of the cross-primer amplification method of Yersinia pestis in border ports. Report of test results. This section applies to the screening test of Yersinia pestis and the laboratory of Yersinia pestis in entry and exit personnel at border ports. Detection.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB 19489 General requirements for laboratory biosafety SN/T 1280-2014 patrol inspection procedures at border ports WS279-2008 Plague Diagnostic Criteria List of pathogenic microorganisms transmitted from humans (Ministry of Health.2006)

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Cross-primer constant temperature amplification technology crossingprimingisothermalamplification; CPA A nucleic acid constant temperature amplification technology, in addition to a DNA polymerase having a strand displacement function in a CPA' constant temperature amplification system (DNAPoly- In addition to merase, it mainly includes cross-primers, stripping primers and detection primers. These oligonucleotide strands can rely on the DNA polymerase The highly active strand displacement property enables the cyclic amplification of deoxyribonucleic acid (DNA) to be continuously realized. 3.2 Cross primer primer Primary primer for cross-amplification, in which the 5' end sequence of the forward primer is identical to the hybridization sequence of the reverse primer, and the reverse primer The 5' end sequence is identical to the hybridization sequence of the forward primer, so the two primers are introduced into each other's hybridization sequence during amplification. Increase the hybridization site of the primer to promote the amplification reaction. 3.3 Stripping primer bumperprimer A short-stranded primer located behind the cross-amplification primer, which functions to extend the amplification primer under the action of a strand-replacement DNA polymerase The chain is peeled off from the template.

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