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SN/T 3567.7-2017: Detection method of crossing priming isothermal amplification. Part 7: Enterovirus 71(EV71)
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Detection method of crossing priming isothermal amplification. Part 7: Enterovirus 71(EV71)
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SN/T 3567.7-2017
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Basic data | Standard ID | SN/T 3567.7-2017 (SN/T3567.7-2017) | | Description (Translated English) | Detection method of crossing priming isothermal amplification. Part 7: Enterovirus 71(EV71) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 13.020 | | Word Count Estimation | 9,998 | | Date of Issue | 2017-07-21 | | Date of Implementation | 2018-03-01 | | Quoted Standard | GB/T 6682; GB 19489 | | Regulation (derived from) | National Quality Inspection (2017) No. 337 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the subjects, test procedures and test results of the detection of enterovirus 71 cross-primers at the border ports. This section applies to the rapid screening test of enterovirus 71 at border ports. | SN/T 3567.7-2017: Detection method of crossing priming isothermal amplification. Part 7: Enterovirus 71(EV71)
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Detection method of crossing priming isothermal amplification.Part 7. Enterovirus 71 (EV71)
People's Republic of China entry and exit inspection and quarantine industry standards
Cross primer constant temperature amplification detection method
Part 7. Enterovirus 71
Part 7. Enterovirus71 (EV71)
Released on.2017-07-21
2018-03-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 3567 "Cross Primer Constant Temperature Amplification Detection Method" is divided into 11 parts.
--- Part 1. General technical procedures;
--- Part 2. Vibrio cholerae;
--- Part 3. E. coli O157.H7;
--- Part 4. Yellow fever virus;
--- Part 5. Salmonella;
--- Part 6. Mycobacterium tuberculosis;
--- Part 7. Enterovirus 71;
--- Part 8. Plasmodium;
--- Part 9. Yersinia pestis;
---Part 10. Bacillus anthracis;
--- Part 11. Shigella.
This part is part 7 of SN/T 3567.
This part is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Hangzhou Yousida Biotechnology Co., Ltd.
The main drafters of this section. Li Zhihui, Chai Hongsen, Tian Hui, Qi Jun, Hu Lin, especially Min.
Cross primer constant temperature amplification detection method
Part 7. Enterovirus 71
1 Scope
This part of SN/T 3567 specifies the subjects and detection procedures for the detection of enterovirus 71 cross-primers at the border crossings.
Sequence and test results report.
This section applies to the rapid screening test of enterovirus 71 at border ports.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB 19489 General requirements for laboratory biosafety
List of pathogenic microorganisms transmitted from humans (Ministry of Health.2006)
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Cross-primer thermostatic amplification technique crossprimingisothermalamplification; CPA
A nucleic acid constant temperature amplification technology, in addition to RNA polymerase (RNAPoly-
In addition to merase, it mainly includes cross-primers, stripping primers and detection primers. These oligonucleotide strands can rely on the RNA polymerase
The highly active strand displacement property enables the cyclic amplification of RNA to be continuously realized.
3.2
Cross primer crossprimer
Primary primer for cross-amplification, in which the 5' end sequence of the forward primer is identical to the hybridization sequence of the reverse primer, and the reverse primer
The 5' end sequence is identical to the hybridization sequence of the forward primer, so the two primers are introduced into each other's hybridization sequence during amplification.
Increase the hybridization site of the primer to promote the amplification reaction.
3.3
Stripping primer bumperprimer
A short-stranded primer located behind the cross-amplification primer, which functions to extend the amplification primer under the action of a strand-replacement DNA polymerase
The chain is peeled off from the template.
3.4
Detection primer detectionprimer
A pair of short-stranded primers located inside the cross-primer, which need to be labeled with a hapten or fluorescein, so that the amplification product carries the target
Remember to use for testing.
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