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SN/T 2423-2010: Determination of thiamphenicol and florfenicol residues in animal derived food. MIA method
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2423-2010 | English | 369 |
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Determination of thiamphenicol and florfenicol residues in animal derived food. MIA method
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SN/T 2423-2010
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Basic data | Standard ID | SN/T 2423-2010 (SN/T2423-2010) | | Description (Translated English) | Determination of thiamphenicol and florfenicol residues in animal derived food. MIA method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X22 | | Classification of International Standard | 67.120.01 | | Word Count Estimation | 14,123 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Quality Inspection (2010) No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the animal-derived foods thiamphenicol and florfenicol inhibiting microbial drug residue detection method. This standard applies to meat, fish, shrimp and milk thiamphenicol and florfenicol drug residue screening test. | SN/T 2423-2010: Determination of thiamphenicol and florfenicol residues in animal derived food. MIA method
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Determination of thiamphenicol and florfenicol residues in animal derived food.MIA method
Exit inspection and quarantine industry standard book People's Republic of China
Foods of animal origin and thiamphenicol
Florfenicol drug residue detection method
Microbial inhibition
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Entry-Exit Inspection and Quarantine Zhanjiang, People's Republic of China.
The main drafters of this standard. Wang Zhiqiang, Li Zhiyong, Xu Longyan, Ling Li, Chen Zonghui, Chen Chunmei.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Foods of animal origin and thiamphenicol
Florfenicol drug residue detection method
Microbial inhibition
1 Scope
This standard specifies the foods of animal origin thiamphenicol and florfenicol drug residues detected microbial inhibition.
This standard applies to meat, fish, shrimp and milk thiamphenicol and florfenicol drug residue screening test.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 equipment and materials
3.1 device
3.1.1 Electronic balance. a sensitivity of 0.01g.
3.1.2 High-speed centrifuge.
3.1.3 High-speed tissue masher.
3.1.4 Vortex.
3.1.5 constant temperature water bath.
3.1.6 vernier caliper. Measuring range 0mm ~ 200mm, precision 0.02mm or inhibiting ring gauge.
3.1.7 sterilized dishes. diameter 90mm, bottom flat glass or plastic disposable sterile petri dish.
3.1.8 circular filter paper. diameter 10mm, thickness of 1.1mm. Japan ToyoRoshiKaisha, Ltd. Those products or equivalent.
3.1.9 50mL centrifuge tube.
3.1.10 Adjustable Pipette. 10μL ~ 100μL, 100μL ~ 1000μL.
3.1.11 thermostat. 37 ℃ ± 1 ℃.
3.1.12 McFarland nephelometer.
3.2 strains and media
3.2.2 Purification and passaging medium (TSA). See Appendix A, section A. Chapter 1.
3.2.3 Calibration with medium. Antibiotic Medium No. 1 (AMI) See Appendix A A. 2.
3.3 Reagents
Chemicals used in the method of analytical grade, test water is distilled or deionized water, test water should comply with GB/T 6682 in
A water provisions.
pH6.0 phosphate buffer solution. Weigh 8.0g potassium dihydrogen phosphate (of KH2PO4) and 2.0g dipotassium hydrogen phosphate (K2HPO4), distillation
Dissolved in water and dilute to 1000mL, 121 ℃ autoclave 15min.
pH6.0 phosphate buffer, and acetonitrile was prepared by 2.3 volume ratio.
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