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| SN/T 1586.2-2008 | English | 359 |
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Identification of Pseudomonas savastanoi pv.phaseolicola (Burkholder) Gardan et al
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SN/T 1586.2-2008
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Basic data | Standard ID | SN/T 1586.2-2008 (SN/T1586.2-2008) | | Description (Translated English) | Identification of Pseudomonas savastanoi pv.phaseolicola (Burkholder) Gardan et al | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 9,927 | | Date of Issue | 2008-09-04 | | Date of Implementation | 2009-03-16 | | Regulation (derived from) | Industry standard filing Notice No. 11 of 2008 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the export plant quarantine bean halo blight bacterium (Pseudomonas savastanoi pv. phase-olicola) quarantine identification. This standard applies to inbound and outbound bean (Phaseolus vulgaris), soybean (Glycine max) seeds of plants such as beans Halo Blight quarantine and identification. | SN/T 1586.2-2008: Identification of Pseudomonas savastanoi pv.phaseolicola (Burkholder) Gardan et al
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Identification of Pseudomonas savastanoi pv.phaseolicola (Burkholder) Gardan et al
Exit inspection and quarantine industry standard book People's Republic of China
Bean Halo blight identification of quarantine
Posted 2008-09-04
2009-03-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This section of the Appendices B and C are normative appendices, Appendix A is an information appendix.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section is drafted. Tianjin People's Republic of China Exit Inspection and Quarantine.
The main drafters of this section. Liu Peng, Cui Tiejun, Liu Fang, Liuyue Ting, Feng Jie, Wang Jincheng.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Bean Halo blight identification of quarantine
1 Scope
Quarantine and identification of bacteria.
Principle 2
2.1 germs taxonomic status and important features
Bean Halo blight (Haloblightofbeans) is a serious disease legume on beans, soybeans, bean halo disease pathogen
Gram-negative, pole whip solitary or raw, can exercise, no sheath, no spores, average size (0.5μm ~ 1.0μm) × (0.4μm ~
1.5μm), metabolic breathing type of organic energy nutrition, catalase positive, arginine hydrolase double negative, oxidase negative. Info reference
See Appendix A.
2.2 Identification of principle
Morphology Bean Halo Blight, biological characteristics, biochemical characteristics, host range, transmission, as well as molecular biological characteristics
Quarantine and identification of the enactment of this standard basis.
3 instruments, appliances
--- Biological microscope;
--- Biological safety cabinet (or clean benches);
--- Autoclave;
--- Small electric grinder;
--- Incubator;
--- Centrifuge;
--- PCR instrument;
--- Electrophoresis equipment;
--- Gel imaging system;
--- Other conventional laboratory equipment, instruments and reagents.
4 Reagents
Unless otherwise indicated in the analysis using only analytical grade reagents and deionized water. PCR reaction system with water double-distilled water. Than other agents
Such as a variety of solutions, buffers and media are specific detection methods are required to prepare shall be appendix. See using commercially available reagents
Instructions.
Hydrogen peroxide (3%), peptone, yeast extract, magnesium sulfate (MgSO4 · 7H2O), sucrose (C12H22O11), dipotassium hydrogen phosphate
(K2HPO4 on), agar, polypeptone, glycerol, ethylenediaminetetraacetic acid (EDTA), tris (hydroxymethyl) aminomethane (Tris), hydrochloric acid, acetic acid,
Potassium dihydrogen phosphate (of KH2PO4), disodium hydrogen phosphate (of NaH2PO4), agarose, ethidium bromide, T Rao q DNA polymerase, 10 × PCR buffer
Liquid, dNTP (2.5mmol/Leach), bromophenol blue and xylene green FF, 2000bpMarker.
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