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SN/T 1586.1-2005: Detection for Curtobacterium flaccumfaciens pv. Flaccumfaciens (Hedges) Collins and Joues
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| SN/T 1586.1-2005 | English | 289 |
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Detection for Curtobacterium flaccumfaciens pv. Flaccumfaciens (Hedges) Collins and Joues
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SN/T 1586.1-2005
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Basic data | Standard ID | SN/T 1586.1-2005 (SN/T1586.1-2005) | | Description (Translated English) | Detection for Curtobacterium flaccumfaciens pv. Flaccumfaciens (Hedges) Collins and Joues | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 9,99 | | Date of Issue | 2005-05-20 | | Date of Implementation | 2005-12-01 | | Regulation (derived from) | 2005 Bulletin No. 8 industry standard | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the entry plant quarantine bean bacterial wilt pathogen detection methods. This standard applies to beans from bacterial wilt disease in countries and regions, beans, green beans, cylinder beans, soybeans and other plants Ting Kuei grass seeds for the quarantine. | SN/T 1586.1-2005: Detection for Curtobacterium flaccumfaciens pv. Flaccumfaciens (Hedges) Collins and Joues
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Detection for Curtobacterium flaccumfaciens pv.Flaccumfaciens (Hedges) Collins and Joues
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Method for detecting bacterial wilt pathogen of kidney bean
Released on.2005-05-20
Implementation of.2005-12-01
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A, Appendix B and Appendix C of this standard are informative annexes.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. 焱 焱, Hong 炜, Ning Yueming, Zhao Jiwen, Zhao Hanqing.
This standard is the first industry standard for entry-exit inspection and quarantine.
Method for detecting bacterial wilt pathogen of kidney bean
1 Scope
This standard stipulates the detection method of bacterial wilt pathogen of bean in the entry phytosanitary.
This standard is applicable to plant species such as kidney beans, mung beans, cowpeas, soybeans and diced grasses from countries and regions where bacterial wilt disease occurs.
Sub-entry quarantine.
2 instruments and reagents
2.1 Equipment
Biological microscope, fluorescence microscope, ultra-clean workbench, biological incubator, balance, high-speed refrigerated centrifuge, autoclave, beaker, triangle
Bottles, measuring cylinders, test tubes, petri dishes, slides, coverslips, alcohol lamps, etc.
2.2 Laboratory equipment
Scalpels, surgical scissors, tweezers, etc.
2.3 Medium formula
2.3.1 Nutritional Glucose Agar Medium (NDA)
Nutritional agar + 1% (mass concentration) glucose.
2.3.2 King's B medium
20 g of peptone, 10 g of glycerol, 1.5 g of dipotassium hydrogen phosphate (K2HPO4), 1.5 g of magnesium sulfate (MgSO4·7H2O), 15 g of agar,
1000 mL of distilled water. pH = 7.2.
20 g anti-foaming agent complex, 2 g sodium pyrophosphate salt, 1 g Lubrol WFlakes surfactant, 1000 mL distilled water.
3 On-site quarantine and sampling
For a batch of beans and mung beans, mung beans, cowpeas, soybeans and diced from the countries and regions where the bacterial wilt disease occurs (see Appendix A, Appendix B)
Plant seeds such as valerian are randomly sampled at a ratio of 0.5% to 5% of the total amount of the imported grass.
On-site quarantine and sampling should focus on the color of the seed epidermis, especially whether the seed coat and the umbilical part have yellow bacterial stickiness.
Glue or bacterial mucus.
4 methods and steps
4.1 Appearance symptoms
4.1.1 Color of the seed
Bean fungus bacterial wilt pathogen is present inside and outside the seeds of soybean and kidney bean. Systemically infested seeds have a thick layer of bacteria under the seed coat
The layer is bright yellow, and the surface-infested seeds have only a little yellow bacterial mucus in the umbilicus. If it is infected by other varieties, it is orange or purple.
color. Once abnormal seeds are found, isolation or indirect isolation of the bacteria is required.
4.1.2 Observation of plant symptoms
After the seed to be planted in the isolation greenhouse or the designated isolation site grows out of the seedling, the performance of the seed is systematically observed, and the seedlings are mainly observed.
Whether the plants and leaves have abnormal performance and abnormalities on the pods. Once an abnormal plant is found, it is necessary to directly isolate the pathogen.
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