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SN/T 1580-2013: Detection and identification of coconut cadang-cadang viroid
Status: Valid SN/T 1580: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1580-2013 | English | 319 |
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Detection and identification of coconut cadang-cadang viroid
| Valid |
SN/T 1580-2013
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| SN/T 1580-2005 | English | 359 |
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Coconut death-like virus detection method
| Obsolete |
SN/T 1580-2005
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PDF similar to SN/T 1580-2013
Basic data | Standard ID | SN/T 1580-2013 (SN/T1580-2013) | | Description (Translated English) | Detection and identification of coconut cadang-cadang viroid | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 12,128 | | Older Standard (superseded by this standard) | SN/T 1580-2005 | | Regulation (derived from) | National quality recognition (2013), No. 464; industry standard filing Notice No. 1 of 2014 (No. 169 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the identification of quarantine phytosanitary quarantine death in coconut-like virus. This standard applies to palm plants (including species of fruit, seedlings and other propagation materials) quarantine identified coconut death | SN/T 1580-2013: Detection and identification of coconut cadang-cadang viroid---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of coconut cadang-cadang viroid
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1580-2005
Coconut quarantine and identification of death-like virus
Issued on. 2013-08-30
2014-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1580-2005 "coconut death viroid detection methods."
This standard compared with SN/T 1580-2005, in addition to editorial changes outside the main technical changes are as follows.
--- Standard name changed to "coconut-like virus quarantine and identification of death";
--- Improve the original standard detection methods;
--- Modify the original standard principle;
--- Modification result determination.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Hainan Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Li Weidong, Liu Fuxiu, Han Yuchun, Zhang Yong, Li Jiyong.
This standard replaces the standards previously issued as follows.
--- SN/T 1580-2005.
Coconut quarantine and identification of death-like virus
1 Scope
This standard specifies the identification of quarantine phytosanitary quarantine in coconut death-like virus.
This standard applies to palm plants (including species of fruit, seedlings and other reproductive material) quarantine and identification of dead coconut-like virus.
Basic information of 2 coconut-like virus death
Name. Coconutcadang-cadangviroid.
Taxonomic position. viroid Viroid, potato spindle tuber viroid families Pospiviroidae, coconut-like virus death
Genus Cocadviroid.
Transmission. coconut seedling death-like virus, mainly through long-distance transmission.
For additional information, see Appendix A. coconut-like virus death
3 PRINCIPLE OF THE METHOD
Genome coconut-like virus death is cyclic and linear, single-stranded nucleic acid is ribonucleic acid (SSRNA), to monomer and dimer covalently linked
Cyclic or linear molecular structure exists. Covalently closed circular rod-like structure, after denaturation treatment into the cyclic molecule, without becoming line
Shaped, circular RNA molecule that is much slower at a concentration of 5% polyacrylamide gel mobility than the linear molecules of the same molecular weight.
RNA Sequence CCCVd been measured. Symptoms plants, genomic features such virus caused as the basis for the development of this standard.
4 equipment, appliances and reagents
4.1 Equipment
PCR amplification, multi electrophoresis, freezing high-speed centrifuges, gel imaging analysis system, oscillators, refrigerator, water bath, electronic analysis
The balance (the amount being 0.1mg), two-dimensional electrophoresis systems.
4.2 Appliances
Micro adjustable pipette (0.01μL ~ 2μL, 1μL ~ 10μL, 10μL ~ 100μL, 10μL ~ 1000μL), adjustable pipette
Head, blades and mortar and the like.
4.3 Reagents
Polyacrylamide gel electrophoresis detection reagents (see Appendix B), RT-PCR detection reagent (see Appendix C).
5 pathogen detection
5.1 Symptom Check
Check the palm fruit plant propagation material and seed, seedlings and other suspicious symptoms. For death-like virus occurred from coconut area
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