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SN/T 1559.3-2005: Protocol of haemadsorption test for African swine fever virus
Status: Obsolete SN/T 1559.3: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1559.3-2005 | English | 239 |
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Protocol of haemadsorption test for African swine fever virus
| Obsolete |
SN/T 1559.3-2005
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| SN/T 1559.3-1995 | English | RFQ |
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3 days [Need to translate]
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(African swine fever virus erythrocyte assay procedures)
| Obsolete |
SN/T 1559.3-1995
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PDF similar to SN/T 1559.3-2005
Basic data | Standard ID | SN/T 1559.3-2005 (SN/T1559.3-2005) | | Description (Translated English) | Protocol of haemadsorption test for African swine fever virus | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 6,646 | | Date of Issue | 2005-02-17 | | Date of Implementation | 2005-07-01 | | Regulation (derived from) | Industry standard filing Notice No. 4 of 2005 (No. 64 overall); National Quality recognize [2010] No. 98 Notice industry standard record No. 5, 2010 (No. 125 overall) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies: red blood assay to detect African swine fever virus Method, This standard applies to: detect tissue or peripheral blood African swine fever virus, | SN/T 1559.3-2005: Protocol of haemadsorption test for African swine fever virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of haemadsorption test for African swine fever virus
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
African swine fever virus red blood cell adsorption test operation procedure
Released on.2005-02-17
2005-07-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard refers to the "Diagnostic Test and Vaccine Standards Manual" (2004 edition) published by the International Organization of Animal Health (OIE).
Red blood cell adsorption test method for diagnosis of pigs.
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China is responsible for drafting.
The main drafters of this standard. You Hong, Xu Rusu, Liu Zhongyong, Wang Pengjun.
This standard is the first industry standard for entry-exit inspection and quarantine.
African swine fever virus red blood cell adsorption test operation procedure
1 Scope
This standard specifies the method for the detection of African swine fever virus by red blood cell adsorption test.
This standard applies to the detection of African swine fever virus in tissues or peripheral blood.
2 Instruments and equipment
2.1 Tissue grinder or masher.
2.2 Centrifuge.
2.3 Carbon dioxide incubator.
2.4 Constant temperature incubator or water bath.
2.5 Microscope, inverted microscope.
2.6 leigton tube (160mm x 16mm).
3 test materials
3.1 0.9% sodium chloride. See Appendix A for the preparation method.
3.2 0.01mol/L pH7.4PBS (phosphate buffer). See Appendix A for the preparation method.
3.3 Penicillin, streptomycin, gentamicin and nystatin. It is advisable to prepare 10000 IU/mL with the above PBS before use.
10 mg/mL, 500 IU/mL and 500 IU/mL solutions.
3.4 Heparin. Prepare 1000 IU/mL with 0.9% sodium chloride before use.
3.5 Cell base medium (such as MEM, etc.). See Appendix A for the preparation method.
3.6 1% pig red blood cells. See Appendix A for the preparation method.
3.7 African swine fever standard strain.
3.8 Materials to be tested. including heparin anticoagulation and tissues such as spleen, tonsil, kidney, lymph nodes. The material to be inspected should be placed at as low temperature as possible.
However, it is stored and transported in a container that is not frozen.
4 method of operation
4.1 Primary leukocyte blood cell adsorption test
4.1.1 Preparation of tissue suspension
The tissue to be examined was ground with a tissue grinder or masher, and 0.01 mol/L pH PBS and antibiotic were added to prepare a 20% group.
Woven suspension (antibiotic content per ml of tissue suspension. penicillin 1000 IU, streptomycin 1 mg, gentamicin 50 IU, nystatin)
50IU), spare.
4.1.2 Preparation of white blood cells
Take healthy pig blood, degauss with glass beads or anticoagulate with heparin (100 IU heparin per ml of pig blood), centrifuge at 700g for 30min, aspirate
Serum or plasma is spared. In addition, leukocytes were aspirated and washed 3 times with cell-based medium, and used.
4.1.3 Preparing cell culture fluid
The above serum or plasma is added to the cell base culture solution to prepare a cell culture solution, and the final concentration of serum or plasma is 10%.
30%, while adding antibiotics.
4.1.4 Culture white blood cells
The leukocytes were suspended in a cell culture medium to have a cell content of 107/mL, and were dispensed into a 160 mm×16 mm leigton tube.
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