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SN/T 1554-2016 English PDF

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SN/T 1554-2016: Quarantine protocol for chicken infectious bursal disease
Status: Valid

SN/T 1554: Evolution and historical versions

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
SN/T 1554-2016	English	329	Add to Cart	3 days [Need to translate]	Quarantine protocol for chicken infectious bursal disease	Valid	SN/T 1554-2016
SN/T 1554-2005	English	319	Add to Cart	3 days [Need to translate]	Protocol of enzyme-linked immunosobent assay for avian infectious bursal disease	Obsolete	SN/T 1554-2005

PDF similar to SN/T 1554-2016

Standard similar to SN/T 1554-2016

GB/T 20365 SN/T 1353 NY/T 909 SN/T 1559 SN/T 1556 SN/T 1555

Basic data

Standard ID	SN/T 1554-2016 (SN/T1554-2016)
Description (Translated English)	Quarantine protocol for chicken infectious bursal disease
Sector / Industry	Commodity Inspection Standard (Recommended)
Classification of Chinese Standard	B41
Word Count Estimation	14,162
Date of Issue	2016-12-12
Date of Implementation	2017-07-01
Regulation (derived from)	State-Quality-Inspection-Accredidation (2016) No.590
Issuing agency(ies)	General Administration of Customs

SN/T 1554-2016: Quarantine protocol for chicken infectious bursal disease

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for chicken infectious bursal disease China's entry-exit inspection and quarantine industry standards Replacing SN/T 1554-2005 Chicken bursal disease quarantine technical specification Published on.2016-12-12 2017-07-01 Implementation China The State Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces SN/T 1554-2005 "Infectious Bursal Disease Enzyme-Linked Immunosorbent Assay Procedure" and SN/T 1554- Compared with.2005, the main technical changes are as follows. --- Increased the isolation and identification of chicken bursal disease virus; --- Increased direct immunofluorescence test; --- Increased reverse transcription-polymerase chain reaction; --- Increased real-time fluorescence reverse transcription-polymerase chain reaction; --- Increased agar gel immunodiffusion test. This standard is based on Chapter 2.3.12 of the OIE Terrestrial Animal Diagnostic Test and Vaccine Standards Manual (2008), Infectious Bursal Disease - Ganpo Luo disease. Please note that some of the contents of this document may involve patents. The issuing agency of this document does not assume responsibility for identifying these patents. This standard is proposed and managed by the National Certification and Accreditation Regulatory Commission. This standard was drafted by. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China and Shenzhen Institute of Inspection and Quarantine. The main drafters of this standard are. Sun Jie, Liao Lishan, Tao Hong, Liu Jianli, Yang Chunhua, Yang Junxing, Tang Jinming, Lin Qingyan, Zeng Shaoling, Zhang Caihong, Qin Zhifeng, Lv Jianqiang, Hua Qunyi, Cao Yifu, Zong Hui, Chen Shuzheng, Zhang Qiang, Yan Zhouyi. The previous versions of the standards replaced by this standard are. ---SN/T 1554-2005. Chicken bursal disease quarantine technical specification

1 Scope

This standard specifies the clinical symptoms and pathological changes of chicken bursal disease, virus isolation and identification, reverse transcription-polymerase chain reaction, real-time Fluorescence reverse transcription-polymerase chain reaction, direct immunofluorescence assay, agar gel immunodiffusion assay, and enzyme-linked immunosorbent assay. This standard applies to the diagnosis and quarantine of Type I bursal disease in chickens entering and exiting the country.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods GB/T 18088 Entry-Exit Animal Quarantine Sampling GB/T 27634-2011 Infectious bursal disease virus nucleic acid detection method

3 Diagnostic Technology

3.1 Clinical diagnosis 3.1.1 Clinical symptoms When some or all of the following symptoms occur, it can be used as one of the basis for initial diagnosis. asphyxia, diarrhea, discharge of white sticky or watery The appetite gradually disappears, and the neck and whole body shudder. The gait of the diseased chicken is unstable, the feather is fluffy, the spirit is wilted, the lying place does not move, the body temperature rises, and the cloaca surrounds. The feathers were contaminated with faeces, severe dehydration, dry claws, sunken eye sockets, and failure to die. See Appendix A for others. 3.1.2 Pathological changes When the following macroscopic lesions appear, it can be used as one of the basis for initial diagnosis. Burst of cysts, severe hemorrhage is purple and black. Such as purple grape-like, bursal edema, wall thickening, round shape, yellowish, wrapped with transparent jelly-like exudate; muscle bleeding, thigh Common streaked or plaque-like hemorrhages in the lateral and thoracic muscles; bleeding or hemorrhages at the junction of the glands and muscles; enlarged kidneys, urate Deposition, ureter dilation. 3.2 Virus isolation and identification 3.2.1 Sample Collection and Processing According to the requirements of GB/T 18088 for sample collection, all water shall meet the GB/T 6682 secondary water and tertiary water requirements. Clinical symptoms of death and death of chickens, necropsy after taking the bursa and spleen, chopped, 1.5 (mass to volume ratio) ratio added antibiotic-containing 0.01mol/L phosphate buffer (PBS, pH 7.2) (see Appendix B for the formulation. Repeated freezing and thawing three times after grinding, and centrifugation at 4000r/min for 10 min. Take the supernatant as a sample to be tested.

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