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SN/T 1476-2004: Protocol of polymerase chain reaction (PCR) for Renibacterium salmoninarum
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1476-2004 | English | 199 |
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Protocol of polymerase chain reaction (PCR) for Renibacterium salmoninarum
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SN/T 1476-2004
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PDF similar to SN/T 1476-2004
Standard similar to SN/T 1476-2004 GB 5009.261 SN/T 5662 SN/T 5480 SN/T 1474 Basic data | Standard ID | SN/T 1476-2004 (SN/T1476-2004) | | Description (Translated English) | Protocol of polymerase chain reaction (PCR) for Renibacterium salmoninarum | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B50 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 6,695 | | Date of Issue | 2004-11-17 | | Date of Implementation | 2005-04-01 | | Regulation (derived from) | 2005 Bulletin No. 3 industry standard | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the technical detected by polymerase chain reaction method salmon renal tuberculosis. This standard applies to salmon renal tuberculosis detection. | SN/T 1476-2004: Protocol of polymerase chain reaction (PCR) for Renibacterium salmoninarum---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of polymerase chain reaction (PCR) for Renibacterium salmoninarum
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
X. faecalis polymerase chain reaction
Operating procedure
Released on November 11,.2004
2005-04-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Shi Xiujie, Liu Wei, Gao Longying, Jiang Yulin.
This standard is the first industry standard for entry-exit inspection and quarantine.
Rhodobacter sphaeroides polymerase chain reaction protocol
1 Scope
This standard specifies methods for the detection of salmonella faecalis by polymerase chain reaction.
This standard applies to the detection of salmon bacillus.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
3 reagents and materials
3.1 T犪狇 enzyme
Store at -20 °C, do not freeze or thaw repeatedly or drastic changes in temperature.
Containing 10 mmol/L each of dCTP, dGTP, dATP, and dTTP.
3.3 Primers
A pair of primers with a concentration of 40 μmol/L and the sequence is.
5'-CAAGGTGAAGGGAATTCTTCCACT-3'
5'-GACGGCAATGTCCGTTCCCGGTTT-3'
A 501 base DNA fragment of p57 protein in Rhizopus sinensis was amplified.
3.4 absolute ethanol
Analytically pure, pre-cooled to -20 °C before use.
3.5 mineral oil
No DNase and RNase are required.
3.6 water
The experimental water is shown in GB/T 6682, where the water used for PCR (including nucleic acid extraction) is treated with DEPC to remove DNase and
RNase.
4 Equipment and equipment
PCR amplification instrument, electrophoresis instrument, micro pipette and tip, UV observation lamp, constant temperature incubator, ordinary refrigerator, low temperature refrigerator, electric homogenization
, centrifuge, centrifuge tube, scissors, tweezers.
5 sampling and sample preparation
5.1 Sampling
Take kidneys or other internal organs with necrotic lesions.
If there is no tissue lesion, the kidney is the priority collection, followed by the body fluid or blood cells of the mature female. Fish eggs take the yolk sac film,
Young fish with a body length of less than 4 cm take the whole fish, and fish with a body length of 4 cm to 6 cm take all the internal organs including the kidney.
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