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SN/T 1447.2-2005 English PDF

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SN/T 1447.2-2005: Pleuropneumoniae Actinobacillus polymerase chain reaction operating procedures
Status: Obsolete
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PDF similar to SN/T 1447.2-2005


Standard similar to SN/T 1447.2-2005

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Basic data

Standard ID SN/T 1447.2-2005 (SN/T1447.2-2005)
Description (Translated English) Pleuropneumoniae Actinobacillus polymerase chain reaction operating procedures
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 10,175
Date of Issue 2/17/2005
Date of Implementation 2005-07-01
Regulation (derived from) Industry standard filing Notice 2005 No. 4 (No. 64 overall)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the Actinobacillus pleuropneumoniae polymerase chain reaction method. This standard applies to Actinobacillus pleuropneumoniae identification and detection.

SN/T 1447.2-2005: Pleuropneumoniae Actinobacillus polymerase chain reaction operating procedures

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Pleuropneumoniae Actinobacillus polymerase chain reaction operating procedures Book of the People's Republic of China Entry and Exit Inspection and Quarantine Actinobacillus pleuropneumoniae polymerase chain reaction Operating procedure Released on.2005-02-17 2005-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix, and Appendix B and Appendix C are informative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Li Shuqing, Hu Yongqiang, Li Jian, Wang Qiaoquan, Chen Zhifei. This standard is the first industry standard for entry-exit inspection and quarantine. Actinobacillus pleuropneumoniae polymerase chain reaction Operating procedure

1 Scope

This standard specifies the polymerase chain reaction method of Actinobacillus pleuropneumoniae. This standard applies to the identification and detection of Actinobacillus pleuropneumoniae.

2 Abbreviations

The following abbreviations apply to this standard. PCR polymerasechainreaction polymerase chain reaction

3 Principle

The specific primers P1/P4 and the universal primer S7/S10 designed for the sequence of Actinobacillus 16SrRNA, can be amplified by composite PCR Fragments of 363 bp and 692 bp were used for the detection of Actinobacillus pleuropneumoniae. In order to improve the sensitivity of detection, two in P1/P4 The specific primer P6/P8 was designed inside the side to form a nested PCR, which can amplify a 223 bp fragment.

4 Equipment and materials

4.1 Instrumentation 4.1.1 PCR instrument. 4.1.2 Electrophoresis apparatus. 4.1.3 Gel imaging system. 4.1.4 Microwave oven. 4.1.5 Adjustable pipette. 4.1.6 Centrifuge. 4.1.7 Homogenizer. 4.1.8 Balance. 4.2 Consumables 4.2.1 PCR reaction tube. 4.2.2 1.5 mL centrifuge tube. 4.2.3 Pipette dripper. 4.3 Reagents 4.3.1 Three steamed water 0.103 MPa sterilization for 15 min. 4.3.2 Tween-20 (Tween-20) 0.103 MPa sterilization for 15 min. 4.3.3 Agarose. 4.3.4 Ethidium bromide. 4.3.5 T犪狇 enzyme.

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