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SN/T 1226-2015 English PDF

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SN/T 1226-2015: Quarantine protocol for fowlpox
Status: Valid

SN/T 1226: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 1226-2015English329 Add to Cart 3 days [Need to translate] Quarantine protocol for fowlpox Valid SN/T 1226-2015
SN/T 1226-2003English239 Add to Cart 2 days [Need to translate] Fowlpox antibody detection methods. Hemagglutination inhibition test Obsolete SN/T 1226-2003

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Basic data

Standard ID SN/T 1226-2015 (SN/T1226-2015)
Description (Translated English) Quarantine protocol for fowlpox
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 14,167
Date of Issue 2015-02-09
Date of Implementation 2015-09-01
Older Standard (superseded by this standard) SN/T 1226-2003; SN/T 1557-2005
Quoted Standard GB/T 6682; GB 19489
Regulation (derived from) State-Quality-Inspection-Accreditation [2015] 59
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the fowl pox quarantine clinical diagnosis, virus isolation and identification, smear microscopy, the agar immunodiffusion test, fluorescent antibody test, hemagglutination inhibition test, polymerase chain reaction quarantine technical requirements. This standard applies to entry and exit quarantine, diagnostic and monitoring fowl pox.

SN/T 1226-2003: Fowlpox antibody detection methods. Hemagglutination inhibition test

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Fowlpox antibody detection methods.Hemagglutination inhibition test Book of the People's Republic of China Entry and Exit Inspection and Quarantine SN/T 1226-2003 Avian pox antibody detection method red blood cell agglutination Inhibition test Released on.2003-05-28 Implementation of.2003-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Lin Qingyan, Lu Tiekang, Fan Wanhong. This standard is the industry standard for inspection and quarantine issued for the first time. SN/T 1226-2003 Avian pox antibody detection method erythrocyte agglutination inhibition test

1 Scope

This standard specifies methods for the detection of anti-fowlpox antibodies in poultry serum using a hemagglutination inhibition test. This standard applies to epidemiological investigation, diagnosis, quarantine and epidemic monitoring of fowl pox.

2 Reference standard

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682-1992 Analytical laboratory water specifications and test methods (eqv ISO 3696.1987)

3 Principle

This standard uses hemagglutination inhibition method. The principle of the method is to use certain viruses or viruses of hemagglutinin to selectively make one or more Animal red blood cells agglutinate, when the virus suspension is first added with specific antibodies, and the amount of this antibody is sufficient to inhibit viral particles or blood In the case of a lectin, the receptor on the surface of the red blood cell cannot be in direct contact with the virus particle or its hemagglutinin. At this time, the agglutination of red blood cells is suppressed. system. In the known poultry pox virus suspension, the poultry serum to be tested is added, and if it contains an anti-fowlpox virus antibody, it can be combined with the fowlpox virus. The hemagglutination of fowlpox virus, the strength of this inhibition is related to the content of fowlpox virus antibodies.

4 instruments and reagents

4.1 Instrument 96-well V-type micro-reaction plates, micro-samplers, micro-oscillators, centrifuges and centrifuge tubes. 4.2 Reagents 4.2.1 Avian pox virus agglutination antigen, anti-fowlpox virus negative, positive control serum. 4.2.2 0.85% sodium chloride, PBS solution, red blood cell preservation solution (alkali solution), 0.5% chicken red blood cell suspension. See Appendix A for the preparation method. 4.2.3 Serum and treatment to be tested. The blood of the animal to be tested is aseptically collected, and the serum is separated by centrifugation and inactivated in a water bath at 56 ° C for 30 min. 4.2.4 The distilled water used for the test is the tertiary water in GB/T 6682-1992.

5 Method of operation

5.1 Hemagglutination test (HA) 5.1.1 Take a clean 96-well V-type micro-reaction plate and operate according to Table 1. Table 1 The pox pox antigen hemagglutination titer is measured in microliters Hole number 1 2 3 4 5 6 7 8 9 10 11 12 Antigen dilution factor 2 4 8 16 32 64 128 256 512 1024 2048 PBS 50 50 50 50 50 50 50 50 50 50 50 50 Different dilution Antigen 50 50 50 50 50 50 50 50 50 50 50 0 0.5% red blood cells 50 50 50 50 50 50 50 50 50 50 50 50 Room temperature (20 ° C ~ 25 ° C) 30min Example of judgment + + + + + + + + - - - - SN/T 1226-2003

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