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SN/T 1224-2012 English PDF

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SN/T 1224-2012: Quarantine protocol for avain mycoplasmosis
Status: Valid

SN/T 1224: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 1224-2012English339 Add to Cart 3 days [Need to translate] Quarantine protocol for avain mycoplasmosis Valid SN/T 1224-2012
SN/T 1224-2003English279 Add to Cart 3 days [Need to translate] Mycoplasma gallisepticum infection antibody detection methods. Rapid serum agglutination test Obsolete SN/T 1224-2003

PDF similar to SN/T 1224-2012


Standard similar to SN/T 1224-2012

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Basic data

Standard ID SN/T 1224-2012 (SN/T1224-2012)
Description (Translated English) Quarantine protocol for avain mycoplasmosis
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 13,196
Older Standard (superseded by this standard) SN/T 1224-2003
Quoted Standard GB/T 6682; GB 19489
Adopted Standard OIE "Manual of Diagnostic Tests and Vaccines for Terrestrial Animals" (2010 edition) Section 2.3.5, " avian mycoplasma disease diagnostic criteria ", NEQ
Regulation (derived from) National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall)
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the clinical diagnosis of avian mycoplasmosis, serological diagnosis and avian Mycoplasma gallisepticum (MG) Isolation and identification methods. This standard applies to avian mycoplasma disease quarantine diagnosis.

SN/T 1224-2012: Quarantine protocol for avain mycoplasmosis

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Quarantine protocol for avain mycoplasmosis People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1224-2003 Technical specifications avian mycoplasma disease quarantine Issued on. 2012-12-12 2013-07-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard and OIE "ManualofDiagnosticTestsandVaccinesforTerrestrialAnimals" (2010 Edition) Section 2.3.5, "avian mycoplasma disease diagnostic criteria for" consistency is not equivalent. Instead of the standard SN/T 1224-2003 "Mycoplasma gallisepticum antibody detection method is rapid serum agglutination test." This standard compared with SN/T 1224-2003, the main technical changes are as follows. --- Added the disease clinical pathological diagnosis content; --- On laboratory diagnostic methods increase pathogen isolation and indirect immunofluorescence (IFA), PCR identification methods, rapid immune colloidal gold Testing, ELISA and hemagglutination inhibition test method. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Hubei Exit Inspection and Quarantine, Huazhong Agricultural University, Shenzhen, the People's Republic of China Entry Inspection and Quarantine Bureau. The main drafters of this standard. Chen Jianjun, Xiao Yun Cai, Dong, Pinghan Li Ye Yi gifted, Zengxian Dong, Yang Wu, Biding Ren, Guo star. This standard replaces the standards previously issued as follows. --- SN/T 1224-2003. Technical specifications avian mycoplasma disease quarantine

1 Scope

This standard specifies the clinical diagnosis of avian mycoplasma disease, and serological diagnosis of avian Mycoplasma gallisepticum (MG) and identification of culture isolated. This standard applies to the diagnosis of avian quarantine mycoplasma disease.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB 19489 General requirements for laboratory biosafety

3 Abbreviations

The following abbreviations apply to this document. AM. Avian mycoplasmosis (avainmycoplasmosis) CRD. chronic respiratory disease (chronicrespiratorydisease) MG. avian Mycoplasma gallisepticum (avian Mycoplasma gallisepticum) (mycoplasmagalisepticum) MS. Mycoplasma synovial fluid (mycoplasmasynoviae)

4 Disease Overview

All kinds of pathogenic avian mycoplasma infection caused diseases are collectively referred to as avian mycoplasmosis (AM), in which the infection caused by the MG most AM Is a common, chronic respiratory disease, also known as chicken (CRD), its etiology, epidemiology, clinical symptoms and pathological changes in Appendix A.

5 Laboratory diagnosis

Isolation of the 5.1 MG 5.1.1 Material preparation 5.1.1.1 testing equipment, equipment. biological microscope, fluorescence microscope incident, carbon dioxide incubator, sterile cotton swabs, slides, pH Precision strip. 5.1.1.2 Reagents. MG broth, MG solid medium, phosphate buffer (0.01mol/L, pH7.2PBS) (see Appendix B). 5.1.2 diseased take and save Diseased collected from live poultry and fresh dead birds, for live poultry from the nasal cavity, oropharynx, eyes, esophagus, trachea, cloaca and intercourse is Sampling. Dead birds from the nasal cavity, infraorbital sinus, trachea, or balloon sampling can also be learned and joint infraorbital sinus washings or exudate conjunctival sac. chicken Embryos from the inner surface of the yolk sac, and balloon oropharyngeal samples. MG small piece of tissue is placed in a liquid medium, the number of swabs stirred vigorously in the medium Times, culture samples as soon as possible. All experimental operation should meet the requirements in GB 19489 General requirements for laboratory biosafety.

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