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SN/T 1225-2003: Protocol of diagnostic method for leucocytozoosis. Microscopic examination
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| SN/T 1225-2003 | English | 289 |
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Protocol of diagnostic method for leucocytozoosis. Microscopic examination
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SN/T 1225-2003
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Basic data | Standard ID | SN/T 1225-2003 (SN/T1225-2003) | | Description (Translated English) | Protocol of diagnostic method for leucocytozoosis. Microscopic examination | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 9,964 | | Date of Issue | 2003-05-28 | | Date of Implementation | 2003-12-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the method of identification leucocytozoon coccidiosis. This standard applies to leucocytozoon echinococcosis epidemiology, diagnosis, quarantine and surveillance. | SN/T 1225-2003: Protocol of diagnostic method for leucocytozoosis. Microscopic examination---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of diagnostic method for leucocytozoosis.Microscopic examination
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Diagnostic method for living with leukocyte disease
Released on.2003-05-28
Implementation of.2003-12-01
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix B of this standard is a normative appendix, and Appendix A is an informative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
Drafters of this standard. Fan Wanhong, Liu Youjun, Zhong Anqing.
This standard is the first published inspection and quarantine industry standard.
Diagnostic method for living with leukocyte disease
1 Scope
This standard specifies methods for the identification of leukocyte disease.
This standard applies to epidemiological investigation, diagnosis, quarantine and epidemic monitoring of leukocytoma.
2 Diagnostic principle
This standard is a microscopic examination of stained blood smears and organ tissue cut (pressure) tablets to confirm the diagnosis of leukocytoma. live
The development of white worms (see Appendix A) includes three stages. schizogulation, gamete reproduction and spore reproduction. The first phase and the second phase
Most of them are done in poultry, and part of the second stage and the third stage are completed in the body of blood-sucking insects. Different species of white worms at home
The life history of poultry is basically the same, and its reproductive stage in the poultry is divided into five periods. Such as from the heart, liver, brain and other organs and muscles of sick birds
It can be confirmed by finding the worms in any of the five different periods of schizont or gamete reproduction in meat or blood.
3 equipment and reagents
3.1 Clean glass slides, coverslips, 75% alcohol cotton balls, sterile injection needles, mortar, brown small-mouth reagent bottles, dyeing cylinders, microscopes.
3.2 Methanol, double distilled water, 2% and 10% Giemsa stain (see Appendix B), neutral pure glycerin, normal saline.
4 method of operation
4.1 Blood test
4.1.1 Preparation of blood smears
Take a few pieces of clean slides without grease, select the slide with a smooth edge as the push piece, hold the slide with the thumb and middle finger of the left hand, and hold the slide with the right hand.
Push the film. First disinfect the lower venules or crown of the poultry with a 75% alcohol cotton ball and use a sterile needle to collect a small drop of blood from the disinfection site.
At the right end of the slide, tilt the push piece by 30 ° C ~ 40 ° C, so that one end is in contact with the slide and placed before the blood drop, pull the push piece backward to make the blood
After the droplet is contacted, after the blood is diffused to form a line, the pusher is gently pushed forward at an equal speed to uniformly spread the blood on the slide.
a film. After the blood piece is naturally dried, drop 2 to 3 drops of methanol onto the blood film for 1 min, fix it, and then stain.
4.1.2 Blood smear staining
Take 10% Giemsa staining solution and add to the fixed blood film for 20min~30min, then rinse with distilled water for 2min~
5 min, dry and microscopic examination. The blood piece can also be erected in 2% Giemsa staining solution overnight, taken out, rinsed with distilled water, and dried.
Microscopic examination.
4.1.3 Microscopy
Red blood cells were deformed under the oil microscope, and a few cells ruptured. There are worms (gametophytes) at different developmental stages in both red blood cells and white blood cells.
A typical gametophyte appears in white blood cells (see Appendix A). The worm body is nearly circular and has a lightly stained nucleus. Host cell nucleus is stained
Deep band, one third of the surrounding body. The entire host cell is nearly circular.
4.2 Organ tissue small nodule compression test
4.2.1 Preparation of organ tissue small nodule compression tablets
Pick a small piece of the diseased tissue of the nodular white spots in the liver, heart muscle, chest muscles, brain or kidney (small grain size), place on a glass slide, drop
Add 2 drops to 3 drops of 1.1 glycerol physiological saline, press it with another glass slide several times to make the tissue block on the sheet thin, the liquid is cloudy, and then cover
Slide, to be examined by microscopy.
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