SN/T 1203: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1203-2010 | English | 359 |
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To edible fats transit qualitative detection method for real-time PCR gene plant ingredients
| Obsolete |
SN/T 1203-2010
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| SN/T 1203-2003 | English | 399 |
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Protocol of the qualitative polymerase chain reaction for detecting genetically modified plant components in edible oil
| Obsolete |
SN/T 1203-2003
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PDF similar to SN/T 1203-2010
Basic data | Standard ID | SN/T 1203-2010 (SN/T1203-2010) | | Description (Translated English) | To edible fats transit qualitative detection method for real-time PCR gene plant ingredients | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X14 | | Classification of International Standard | 67.200 | | Word Count Estimation | 9,93 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Older Standard (superseded by this standard) | SN/T 1203-2003 | | Quoted Standard | GB/T 6682; GB 27025; GB/T 19495.2; SN/T 1204 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the transgenic plant ingredients edible fats real-time PCR qualitative detection method. This standard applies to edible fats transgenic plant ingredients qualitative real-time fluorescence PCR assay. This standard does not apply to animal fats transgenic plant ingredients qualitative real-time fluorescence PCR assay. | SN/T 1203-2010: To edible fats transit qualitative detection method for real-time PCR gene plant ingredients
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To edible fats transit qualitative detection method for real-time PCR gene plant ingredients
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1203-2003
Edible oils and fats transgenic plant components
Real-time PCR for qualitative detection method
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1203-2003 "edible oil plant genetic component Qualitative PCR Detection of transit."
This standard compared with SN/T 1203-2003, the main technical changes are as follows.
--- Re-regulate the scope of use of vegetable oil;
--- Redefined the nucleic acid extraction method of edible oils and fats;
--- Replace conventional PCR detection PCR and fluorescent PCR (incorporation assay) for the real-time fluorescence PCR;
--- Adjustment of the qualitative PCR primers, probes sequences;
--- Redefined judgment based on test results.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Guangdong Exit Inspection and Quarantine, Beijing Wanger Biotechnology Co., Ltd.
The main drafters of this standard. Li Danning, east Wei, Xie force, Zhong Yuqing, Zhongguo Qiang, Cai Ying, Wan Yu Ping, Dong Jie, Deng Hong Ling, Ma Jun, Zhang Jun,
Chen source tree, Xie Pei heart.
This standard replaces the standards previously issued as follows.
--- SN/T 1203-2003.
Edible oils and fats transgenic plant components
Real-time PCR for qualitative detection method
1 Scope
This standard specifies the composition of plant gene real-time PCR qualitative detection method of edible oil transit.
This standard applies to edible oils transgenic plant components in real-time fluorescence Qualitative PCR Detection.
This standard does not apply to animal fats transgenic plant components in real-time fluorescence Qualitative PCR Detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 6682 analytical laboratory use specifications and test methods
GB/T 27025 testing and calibration laboratories General requirements
GB/T 19495.2 technical requirements for GMO testing laboratories
SN/T 1204 GMO plants and their processed products in real-time PCR qualitative test methods
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
The following terms and definitions apply to this document.
3.1.1
DNAextractionandpurification DNA extraction and purification of
DNA is released from the various components of the test sample, followed by removal of the purified PCR DNA reaction inhibitor.
3.1.2
Exogenous gene exogenousgene
Use of other bio-genetic bioengineering technology transferred, so that the performance of the new biological species biological traits.
3.2 Acronyms
The following abbreviations apply to this document.
EPSPS (5-enolpyruvylshkimate-3-phosphatesynthasegene). 5- shikimate-3-phosphate synthase gene
FAM (6-carboxyfluorescein). 6- carboxyfluorescein
NOS (terminatorofnopalinesynthasegene). nopaline synthase gene terminator
TAMRA (carboxy-tetramethyl-rhodamine). carboxy tetramethylrhodamine
tRNALeu. chloroplast RNA leucine transporter gene
Principle 4
This standard relates to edible oil is oil crops processing products, usually at room temperature, an oily liquid, oil pressing, extraction process
, Part of the nucleic acid and other polar group with the destruction of oil crops branch cells into the oil. Using appropriate nucleic acid extraction technology, access to suitable
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