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SN/T 1199-2010: Protocol of the polymerase chain reaction for detecting genetically modified components in cotton
Status: Valid SN/T 1199: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1199-2010 | English | 259 |
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Protocol of the polymerase chain reaction for detecting genetically modified components in cotton
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SN/T 1199-2010
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| SN/T 1199-2003 | English | 399 |
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Protocol of the polymerase chain reaction for detecting genetically modified components in cotton
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SN/T 1199-2003
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PDF similar to SN/T 1199-2010
Basic data | Standard ID | SN/T 1199-2010 (SN/T1199-2010) | | Description (Translated English) | Protocol of the polymerase chain reaction for detecting genetically modified components in cotton | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B32 | | Classification of International Standard | 65.020 | | Word Count Estimation | 10,114 | | Date of Issue | 2010-05-27 | | Date of Implementation | 2010-12-01 | | Older Standard (superseded by this standard) | SN/T 1199-2003 | | Quoted Standard | GB 5491; GB/T 6682; GB/T 10360; GB/T 19495.1; GB/T 19495.2; GB/T 19495.3-2004 | | Regulation (derived from) | National Quality Inspection (2010) 290; industry standard filing Notice 2010 No. 10 (No. 130 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the genetically modified cottonseed PcR column qualitative measurement methods. This standard applies to cottonseed and semi-finished products in the anti- lepidopteran cotton SGK32l, SGK9708, GKl, GK2, GK3, GK5, GKl2, GKl9, GK22, GK95-l GMO detection and ordinary PCR screening of transgenic cotton crystal MON531, MONl445 and MONl5985 real-time PCR identification of strains. | SN/T 1199-2010: Protocol of the polymerase chain reaction for detecting genetically modified components in cotton
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of the polymerase chain reaction for detecting genetically modified components in cotton
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1199-2003
GMO cotton qualitative PCR test methods
Issued on. 2010-05-27
2010-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1199-2003 "Genetically Modified Cotton Qualitative PCR detection method."
This standard compared with SN/T 1199-2003, the main technical changes are as follows.
--- Standardized terminology and way of expression;
--- Increase the range "of genetically modified cotton line MON531, MON1445 and real-time PCR strains MON15985
Identification ";
--- Terminology and abbreviations reference GB/T 19495.1 "GMO testing laboratories technical requirements" provisions;
--- Of cotton DNA extraction, using some of CTAB method (2003 version 6.4.1 and 6.4.2; 9.1.1 Excerpts) mining
Method GB/T 19495.3 prescribed; 9.1.2 Where the increase of the sample is not available for the method in accordance with 9.2.1
GB/T 19495.3 specified in the appropriate way to do;
--- Food in nucleic acid quantification (2003 Version 6.5; 9.2 edition) using the method GB/T 19495.3 prescribed;
--- Increasing the real-time PCR method, and the result is determined in step (2003 edition, Chapter 7; edition 9.5.2) corresponding increase
Plus real-time PCR assay results to determine the method;
--- Conventional PCR amplification reaction system, the reaction cycle number and the result of the judgment (2003 edition 6.6.1,6.6.2,6.6.3 Table 2
And Table 3; Excerpts 8.5.1, Table A.1, Table A.3 and Table A.4) way of expression to rearrange and modify the confirmatory test
method.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Shanghai Exit Inspection and Quarantine.
The main drafters of this standard. Liu Xuan, Liu Wei, He Yan, Panliang Wen, Zhao Weidong, Kit Cheng, Liuyue Ting.
This standard was first published in 2003, in 2010 the first amendment.
GMO cotton qualitative PCR test methods
1 Scope
This standard specifies the qualitative PCR detection of genetically modified cottonseed.
This standard applies to cotton seed and semi-finished products in the anti-lepidopteran cotton SGK321, SGK9708, GK1, GK2, GK3, GK5,
GK12, GK19, GK22, GK95-1 GMO ordinary PCR screening assays and transgenic cotton line MON531, MON1445
Realtime PCR and MON15985 strain identification.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member, undated references, the latest edition (including any amendments) applies to this document.
GB 5491 grain and oilseeds testing for sampling, and sample method
GB/T 6682 analytical laboratory use specifications and test methods
GB/T 10360 for sampling oilseed residues
GB/T 19495.1 GMO testing - General requirements and definitions
GB/T 19495.2 technical requirements for GMO testing laboratories
GB/T 19495.3-2004 GMO detection of nucleic acid extraction and purification method
3 Terms and abbreviations
Terms and abbreviations GB/T 19495.1 defined apply to this document.
Principle 4
After extraction of the sample DNA, for GM cottonseed exogenous gene designed primer and probe sequences by conventional PCR, special
Specifically amplify DNA fragments of exogenous gene, and based on PCR determine whether the sample contains genetically modified ingredients. To carry out strain KAM
Given, through the real-time PCR technology to determine whether the sample contains a strain.
5 Apparatus appliances
5.1 cycler, electrophoresis, electrophoresis tank, gel imaging analysis system, UV-visible spectrophotometer, balance (and a sense of the amount of 1mg
0.1mg), autoclaves, cryogenic refrigerators, refrigerated centrifuge, water bath, microwave, real-time PCR instrument, clean benches,
Ultra-pure water, Vortex.
5.2 micro pipette (2.5μL, 10μL, 20μL, 100μL, 200μL, 1000μL), PCR reaction tube (200μL, 500μL),
Eppendorf tubes (1.5mL and 2.0mL).
6 Reagents
Unless otherwise specified, other reagents were of analytical grade or biochemical reagents, water as a water according to GB/T 6682 regulations.
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