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SN/T 1182.2-2004 English PDF

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SN/T 1182.2-2004: Haemagglutination-inhibition test for avian influenza
Status: Obsolete
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SN/T 1182.2-2004English279 Add to Cart 3 days [Need to translate] Haemagglutination-inhibition test for avian influenza Obsolete SN/T 1182.2-2004

PDF similar to SN/T 1182.2-2004


Standard similar to SN/T 1182.2-2004

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Basic data

Standard ID SN/T 1182.2-2004 (SN/T1182.2-2004)
Description (Translated English) Haemagglutination-inhibition test for avian influenza
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 65.020.30
Word Count Estimation 7,785
Date of Issue 6/1/2004
Date of Implementation 12/1/2004
Regulation (derived from) State-Quality-Inspection-accreditation (2010) 582
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies test methods using micro-hemagglutination test for detection of avian influenza antibodies inhibited; This standard applies to poultry bird flu quarantine, surveillance and epidemiology; .

SN/T 1182.2-2004: Haemagglutination-inhibition test for avian influenza

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Haemagglutination-inhibition test for avian influenza Book of the People's Republic of China Entry and Exit Inspection and Quarantine Avian influenza micro-erythrocyte agglutination inhibition test Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Xu Haini, Xue Jingshan, Pan Wenbo, Liao Xiuyun, Zhou Sibo, Wang Xiaoyu. This standard is the first industry standard for entry-exit inspection and quarantine. Avian influenza micro-erythrocyte agglutination inhibition test

1 Scope

This standard specifies test methods for the detection of avian influenza antibodies using a micro-erythrocyte agglutination inhibition assay. This standard applies to poultry avian influenza quarantine, epidemic monitoring and epidemiological investigation.

2 reagents and materials

2.1 Reagent purity All chemical reagents were of analytical grade. See Appendix A for the preparation method. 2.3 red cell mud See Appendix A for the preparation method. 2.4 1% chicken red blood cell suspension Chicken red cell mud was diluted to a 1% suspension with PBS. 2.5 standard antigen Standard antigens such as avian influenza H5, H7, H9. 2.6 standard serum Standard positive serum and standard negative serum such as avian influenza H5, H7, H9. 2.7 serum to be tested 2.7.1 Aseptic blood is taken from the animal. After coagulation, the serum is centrifuged and stored at 4 ° C or -20 ° C for later use. 2.7.2 The serum of the remaining birds except chicken should be determined first to determine the presence of non-specific agglutination. For specific agglutination, serum is performed as follows Treatment. add 25 μL of chicken red cell mud per 0.5 mL serum, gently shake, let stand for 30 min, centrifuge at.2000 r/min for 2 min to 5 min, red The cells were agglomerated and the adsorbed serum was taken for examination. 2.8 Equipment Plate balance, analytical balance, centrifuge, V-type micro-coagulation plate, micro-oscillator, adjustable pipette, incubator, etc.

3 method of operation

3.1 micro blood coagulation test The micro blood coagulation test is shown in Table 1.

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