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SN/T 1182-2010: Quarantine protocol for avian influenza
Status: Valid SN/T 1182: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1182-2010 | English | 709 |
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Quarantine protocol for avian influenza
| Valid |
SN/T 1182-2010
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| SN/T 1182.1-2003 | English | 239 |
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Detection antibodies against avian influenza virus. Protocol of agar gel immuno-diffusion test
| Obsolete |
SN/T 1182.1-2003
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PDF similar to SN/T 1182-2010
Basic data | Standard ID | SN/T 1182-2010 (SN/T1182-2010) | | Description (Translated English) | Quarantine protocol for avian influenza | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 27,241 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Older Standard (superseded by this standard) | SN/T 1182.1-2003; SN/T 1182.2-2004 | | Quoted Standard | GB/T 19438.1; GB/T 19438.2; GB/T 19438.3; GB/T 19438.4; GB 19489 | | Adopted Standard | WHO/CDS/CSR/NCS/2002.5 Rev.1, NEQ | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of poultry and its products avian flu quarantine specifications. This standard applies to bird flu quarantine. | SN/T 1182.1-2003: Detection antibodies against avian influenza virus. Protocol of agar gel immuno-diffusion test
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection antibodies against avian influenza virus.Protocol of agar gel immuno-diffusion test
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Avian influenza antibody detection method
Agar immunodiffusion test
Released on.2003-03-17
2003-09-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard is based on OIE/Chapter 2.1.14, 4thedition,.2000, Manual on Diagnostic Methods and Vaccine Standards (Manualofstand-
Ardsfordiagnostictestsandvaccines) drafted.
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted by the Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Pan Wenbo, Xue Jingshan, Xu Haini, Liao Xiuyun.
This standard is the industry standard for inspection and quarantine issued for the first time.
Avian influenza antibody detection method agar immunodiffusion test
1 Scope
This standard specifies the operating procedures for avian influenza agar immunodiffusion test.
This standard applies to the detection of avian influenza (AI) virus antibodies in avian serum. Mainly applicable to quarantine and epidemic situation of poultry avian influenza
Monitoring and epidemiological investigations.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682-1992 Analytical laboratory water specifications and test methods
3 Principle
The molecular weight of antigens and antibodies is generally below.200,000 Daltons, and antigens and their specific antibodies are low in the gel from high concentration areas.
When the concentration region diffuses, the resistance is small and is basically in the form of free diffusion. Due to the molecular weight, structure, shape and electricity of different antigen molecules
The charge is different, so the diffusion coefficient is different and the diffusion speed in the gel is different. When the antigen and the corresponding antibody are diffused in the gel
When they meet, an antigen-antibody complex is formed, and if the ratio of the two is appropriate at the meeting, the largest complex is formed. Due to the increased molecular weight of the complex
Large, the particles increase, and thus no longer continue to diffuse to produce a precipitate, appearing in the form of a line or a band, and the formation of a line or band is called immunoprecipitation.
Line or immunoprecipitation zone, therefore, whether the presence of specificity in the serum to be tested can be determined based on whether a sedimentation line appears between the antigen and the test serum.
antibody.
4 Material preparation
4.1 Reagents. sodium dihydrogen phosphate (NaH2PO4·2H2O), disodium hydrogen phosphate (Na2HPO4·12H2O), sodium chloride (NaCl), azide
Sodium, agarose, were of analytical grade.
4.2 Agar diffusion test antigen, standard positive serum. supplied by the designated unit. Use according to the instructions.
4.3 Equipment. straw, measuring cylinder, plate (size. 90mm × 15mm), triangular bottle, metal punch with outer diameter of about 5mm, micro pipette
And micro tips, observation lights, etc.
4.4 Serum to be tested. Serum is collected by routine blood collection. There should be no hemolysis and no bacterial contamination. Store at 4 °C.
5 Method of operation
5.1 Test water
The water used in this standard should meet the specifications of the third grade water (three steamed water) in GB/T 6682-1992.
5.2 Preparation of agar plates
5.2.1 See Appendix A for the preparation of the agar solution.
5.2.2 Plate. When the agar solution is naturally cooled to about 56 ° C, a 90 mm diameter clean and sterilized plate is placed on a horizontal platform, each
Add 15mL to the plate, make the gel thickness 2mm ~ 3mm, cover the plate cover, after the natural cooling and solidification, put the plate into the wet box, 4 ° C
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