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SN/T 1181-2010: Quarantine protocol for foot and mouth disease
Status: Valid SN/T 1181: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1181-2010 | English | 599 |
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Quarantine protocol for foot and mouth disease
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SN/T 1181-2010
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| SN/T 1181.1-2003 | English | 279 |
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Detection antibodies against foot and mouth disease virus infection-associated antigen. Protocol agar gel immuno-diffusion test
| Obsolete |
SN/T 1181.1-2003
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PDF similar to SN/T 1181-2010
Basic data | Standard ID | SN/T 1181-2010 (SN/T1181-2010) | | Description (Translated English) | Quarantine protocol for foot and mouth disease | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 23,273 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Older Standard (superseded by this standard) | SN/T 1181.1-2003; SN/T 1181.2-2003; SN/T 1181.3-2003 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the FMD virus isolation and identification, enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, fluorescence reverse transcription polymerase chain reaction, virus neutralization test, the solid phase competitive enzyme-linked immunosorbent assay, liquid phase blocking ELISA, and agar immunodiffusion test specifications. This standard applies to FMD quarantine. | SN/T 1181.1-2003: Detection antibodies against foot and mouth disease virus infection-associated antigen. Protocol agar gel immuno-diffusion test
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection antibodies against foot and mouth disease virus infection-associated antigen.Protocol agar gel immuno-diffusion test
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Foot and mouth disease virus infection antibody detection method
Agar immunodiffusion test
Released on.2003-03-17
2003-09-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard is based on OIE/Chapter 2.1.1, 4thedition,.2000, Diagnostic Test and Vaccine Standards Manual (Manualofstand-
ardsfordiagnosticTestsandVaccines) drafted.
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Xu Haini, Xue Jingshan, Pan Wenbo, Liao Xiuyun.
This standard is the industry standard for inspection and quarantine issued for the first time.
Foot and mouth disease virus infection antibody detection method
Agar immunodiffusion test
1 Scope
This standard specifies the operating procedures for agar immunodiffusion test of foot-and-mouth disease virus infection-related antigens.
This standard is applicable to the detection of antibodies against foot-and-mouth disease virus-associated antigen (VIA) in susceptible animal serum, and is applicable to all types of foot-and-mouth disease.
Quarantine (no type specificity), epidemic surveillance and epidemiological investigation.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682-1992 Analytical laboratory water specifications and test methods
3 Abbreviations
The following abbreviations apply to this standard.
3.1
Agar immunodiffusion test.
3.2
Foot and mouth disease.
3.3
The virus infects the relevant antigen.
3.4
Trishydroxymethylaminomethane-hydrochloric acid solution.
4 Principle
The antigen and its specific antibody have little resistance when diffused from the high concentration region to the low concentration region in the gel, and are basically freely expanded.
Form of dispersion. Since different molecular molecules differ in molecular weight, structure, shape, and charge amount, their diffusion coefficients are different. When antigen and corresponding
After the antibodies are diffused, they meet in the gel to form an antigen-antibody complex. If the ratio of the two is appropriate at the meeting, the largest complex is formed.
As the molecular weight of the composite increases, the particles increase, and thus do not continue to diffuse to form a precipitate, which exhibits a linear or band shape, and forms a linear shape.
Or a band-like "specific barrier" is called an immunoprecipitation line or an immunoprecipitation zone. Agar immunodiffusion test based on standard antigen and test serum
Whether a sedimentation line appears between them determines whether a specific antibody is present in the serum to be tested.
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