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SN/T 1181.2-2003: Detection antibodies against foot and mouth disease virus. Protocol of micro-serum neutralization test
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Detection antibodies against foot and mouth disease virus. Protocol of micro-serum neutralization test
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SN/T 1181.2-2003
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Basic data | Standard ID | SN/T 1181.2-2003 (SN/T1181.2-2003) | | Description (Translated English) | Detection antibodies against foot and mouth disease virus. Protocol of micro-serum neutralization test | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 8,859 | | Date of Issue | 2003-03-17 | | Date of Implementation | 2003-09-01 | | Adopted Standard | OIE 2.1.1-2000; NEQ | | Regulation (derived from) | State inspection recognized [2010] No. 582 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | SN/T 1181.2-2003: Detection antibodies against foot and mouth disease virus. Protocol of micro-serum neutralization test
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Detection antibodies against foot and mouth disease virus.Protocol of micro-serum neutralization test
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Foot and mouth disease virus antibody detection method
Trace serum neutralization test
Released on.2003-03-17
2003-09-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the relevant content of the OIE Diagnostic Test and Vaccine Standards Manual, Chapter 4, Chapter 2.1.1 of.2000.
Appendix A and Appendix B of this standard are standard informative annexes.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Yang Su, Xue Jingshan, Lu Hong, Feng Jiawang.
This standard is the industry standard for inspection and quarantine issued for the first time.
Foot-and-mouth disease virus antibody detection method, trace serum neutralization test
1 Scope
This standard specifies the test method for the neutralization of foot-and-mouth disease virus.
This standard applies to the detection of neutralizing antibody levels of foot-and-mouth disease virus.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682-1992 "Analysis laboratory water specifications and test methods"
3 Abbreviations
The following abbreviations apply to this standard.
3.1
CPE
Caused by cytopathic effects.
3.2
TCID50
Half of the amount of cell infection.
3.3
FMD
Foot and mouth disease.
4 Principle
The specific serum neutralizing antibody binds to the virus, which can make the virus lose its ability to infect sensitive cells, thus preventing the virus from spreading.
Colonization. This reaction is manifested not only as a virus can only be neutralized by the corresponding immune serum, but also in neutralizing a certain amount of virus,
Must have a certain titer of immune serum. The neutralization test specified in this standard is based on quantitative virus dilution serum, when serum can prevent quantification
When the virus is 50% cytopathogenic, its serum dilution is its potency. Therefore, the neutralization test is a quantitative determination of foot-and-mouth disease blood.
The method of clearing antibodies.
5 reagents and materials
5.1 The water used in this standard shall comply with the specifications for tertiary water (three distilled water) in GB/T 6682-1992.
5.2 Refer to Appendix A for the reagent formulations used in this standard.
5.3 Unless otherwise specified, all reagents in this standard refer to analytical reagents.
5.4 Reagents and glassware used in this standard are autoclaved except for special regulations.
5.5 Virus O, A, C, SAT1, SAT2, SAT3 and AsiaI type foot-and-mouth disease cytotoxicity purified by plaque method and serologically identified
As the standard toxicity for each type of serum antibody detection.
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