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SN/T 1119-2002 English PDF

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SN/T 1119-2002: Identification of bovine, sheep and goat derived material in import animal derived feedstuff. PCR method
Status: Obsolete
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SN/T 1119-2002English999 Add to Cart 5 days [Need to translate] Identification of bovine, sheep and goat derived material in import animal derived feedstuff. PCR method Obsolete SN/T 1119-2002

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Basic data

Standard ID SN/T 1119-2002 (SN/T1119-2002)
Description (Translated English) Identification of bovine, sheep and goat derived material in import animal derived feedstuff. PCR method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B46
Word Count Estimation 25,265
Date of Issue 2002-05-20
Date of Implementation 2002-11-01
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the import of animal feed cattle-derived ingredients detection sampling, sample preparation, PCR method, restriction endonuclease digestion reaction method. This standard applies to animal feed qualitative detection of cattle-derived ingredients.

SN/T 1119-2002: Identification of bovine, sheep and goat derived material in import animal derived feedstuff. PCR method


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of bovine, sheep and goat derived material in import animal derived feedstuff.PCR method People's Republic of China entry and exit inspection and quarantine industry standards Import and export of animal-derived feedstock People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Content

Foreword III Introduction IV 1 Scope 1 2 Terms, definitions and abbreviations 1 3 Sampling and sample preparation 2 4 Determination method 3 5 results and judgment 4 6 Waste treatment and pollution prevention measures 4 Appendix A (Normative) Confirmation Test - PCR Amplification Product Sequencing 5 Appendix B (Normative) Measures to prevent cross-contamination during testing 6 Reference 7

Foreword

Appendix A and Appendix B of this standard are normative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. China Import and Export Commodity Inspection Technology Research Institute, Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Xu Baoliang, Yang Baohua, Wang Jing, Cao Jijuan, and Bian Qingru. This standard is the first industry standard for entry-exit inspection and quarantine.

Introduction

Bovine spongiform encephalopathy and scrapie can be transmitted through edible diseased animal tissues, so the use of animal-derived feed containing bovine and sheep-derived components, Straight is considered to be the main way to spread bovine spongiform encephalopathy and scrapie. It is forbidden to produce animal-derived feed containing cattle and sheep-derived components in infected areas. Production, distribution and use have become the main means to prevent bovine spongiform encephalopathy, scrapie infection and epidemics. According to the specificity of the genetic material of cattle and sheep, the PCR method can be used to detect the bovine and sheep-derived components in animal-derived feed. Measurement of the method The lower limit is 0.125%. Import and export of animal-derived feedstock  range This standard stipulates the sampling, sample preparation, PCR method and restriction endonuclease digestion of bovine and sheep-derived components in imported animal-derived feed. Reaction method. This standard applies to the qualitative detection of bovine and sheep-derived components in animal-derived feed.  Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this standard. . A feed containing animal ingredients. . Bovine specific DNA fragment. . Sheep specific DNA fragment. . Polymerase chain reaction, referred to as PCR. Using two (20 to 24 nucleotides) oligonucleotides as primers for the reaction, these two The sequence of the nucleotide primer should not have a complementary effect. But they can be separated from the two strands of the DNA to be tested, called the template, at a certain point. Complementary. The reaction solution consists of a reaction buffer containing magnesium ions, four kinds of single nucleotides (dNTPs), template DNA, and primers. In DNA A DNA fragment between two complementary sites is synthesized by polymerase catalysis by changes in temperature (DNA denaturation, annealing, and extension). such The reaction is repeated to allow amplification of the DNA fragment produced in the first cycle. After 25 to 30 cycles, the amplification factor reached 106. . Abbreviations PCR. polymerasechainreaction, referred to as PCR. DNA. deoxyribonucleic acid, deoxyribonucleic acid. dNTP. deoxyribonucleosidetriphosphate, deoxynucleotide triphosphate. dATP. deoxyadenosinetriphosphate, deoxyadenosine triphosphate. dCTP. deoxycytidinetriphosphate, deoxycytidine triphosphate. dGTP. deoxyguanosinetriphosphate, deoxyguanosine triphosphate. dTTP. deoxythymidinetriphosphate, deoxythymidine triphosphate. dUTP. deoxyuridinetriphosphate, deoxyuridine triphosphate. UDG. uracil DNAglycosylase, uracil DNA-glycosylase. Bp. basepair, base pair.

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