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SN/T 1059.4-2005 English PDF

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SN/T 1059.4-2005: Inspection of Escherichia coli in food for import and export. Glutamate decarboxylase assay
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
SN/T 1059.4-2005	English	609	Add to Cart	4 days [Need to translate]	Inspection of Escherichia coli in food for import and export. Glutamate decarboxylase assay	Valid	SN/T 1059.4-2005

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Basic data

Standard ID	SN/T 1059.4-2005 (SN/T1059.4-2005)
Description (Translated English)	Inspection of Escherichia coli in food for import and export. Glutamate decarboxylase assay
Sector / Industry	Commodity Inspection Standard (Recommended)
Classification of Chinese Standard	C53;X09
Classification of International Standard	67.040
Word Count Estimation	19,175
Date of Issue	2005-02-17
Date of Implementation	2005-07-01
Quoted Standard	SN 0330
Regulation (derived from)	Industry standard filing Notice 2005 No. 4 (No. 64 overall)
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary	This standard specifies the import and export of E. coli in food sampling and testing methods. This standard applies to imports of frozen meat products, milk and dairy products, frozen vegetables, dehydrated vegetables, water, vinegar, non-alcoholic beverages, walnut, tea, and other foods E. coli health inspection.

SN/T 1059.4-2005: Inspection of Escherichia coli in food for import and export. Glutamate decarboxylase assay

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of Escherichia coli in food for import and export.Glutamate decarboxylase assay Book of the People's Republic of China Entry and Exit Inspection and Quarantine Method for testing E. coli in imported and exported foods Glutamate decarboxylase Released on.2005-02-17 2005-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Content

Foreword I 1 Scope 1 2 Normative references 1 3 Terms and Definitions 1 4 sampling 1 5 Determination method 1 5.1 Principle of the method 1 5.2 Reagents and materials 1 5.3 Instruments and Equipment 2 5.4 Sample preparation and preservation 2 5.5 Determination step 2 5.6 Results determination and reporting 2 Appendix A (Normative) Media and Reagents 3 Appendix B (informative appendix) Calculation of relative centrifugal force 4 Appendix C (Normative) The most approximate value (MPN) of the 1g (mL) sample is shown in Table 5.

Foreword

Appendix A and Appendix C of this standard are normative appendices, and Appendix B is an informative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shanxi Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Li Weihua, Fu Ying, Lian Huifeng, Zhang Jianjun, Gong Hongxia, Song Jie. This standard is the industry's first entry and exit inspection and quarantine standards. Method for testing E. coli in imported and exported foods Glutamate decarboxylase

1 Scope

This standard specifies the sampling and testing methods for E. coli in imported and exported foods. This standard applies to import and export of frozen meat products, milk and dairy products, frozen vegetables, dehydrated vegetables, water, vinegar, non-alcoholic beverages, walnuts, insurance Examination of E. coli in foods such as health tea.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. General rules for microbiological examination of SN 0330 export foods

3 Terms and definitions

The following terms and definitions apply to this standard. 3.1 Also known as Escherichia coli, fermented lactose produces acid and produces gas.吲哚 and methyl red (MR) test positive, V-P test negative, citrate Negative test, glutamate decarboxylase (GAD) test was positive. 3.2 An enzyme that promotes the decarboxylation of glutamic acid. E. coli can specifically produce this enzyme, promote the decarboxylation of glutamic acid, and produce gamma-aminobutyric acid and CO2. 3.3 A statistical method that represents an estimate of 95% confidence limits that can be used to quantify the number of bacteria in a population.

4 sampling

Execute according to SN 0330 regulations.

5 Determination method

5.1 Principle of the method Escherichia coli produces glutamate decarboxylase in an acidic environment, which decomposes glutamic acid in the GAD reaction solution to produce gamma-aminobutyric acid and Carbon dioxide (different from other bacteria of Enterobacteriaceae) changes the pH of the reaction solution, causing a change in the color of the indicator in the reaction solution. 5.2 Reagents and materials 5.2.1 Standard strain

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