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SN/T 1059.2-2002: Total coliform and escherichia coli counts in food for import and export. Membrane filter/MUG method
Status: Obsolete
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1059.2-2002 | English | 239 |
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Total coliform and escherichia coli counts in food for import and export. Membrane filter/MUG method
| Obsolete |
SN/T 1059.2-2002
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Basic data | Standard ID | SN/T 1059.2-2002 (SN/T1059.2-2002) | | Description (Translated English) | Total coliform and escherichia coli counts in food for import and export. Membrane filter/MUG method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53;X09 | | Word Count Estimation | 6,68 | | Date of Issue | 1/16/2002 | | Date of Implementation | 6/1/2002 | | Quoted Standard | SN 0330-1994 | | Adopted Standard | AOAC-1995, NEQ | | Regulation (derived from) | State-Quality-Inspection-accreditation (2010) 582 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | SN/T 1059.2-2002: Total coliform and escherichia coli counts in food for import and export. Membrane filter/MUG method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Total coliform and escherichia coli counts in food for import and export.Membrane filter/MUG method
People's Republic of China entry and exit inspection and quarantine industry standards
Coliform and E. coli counts in imported and exported foods
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is an appendix to the standard.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted by the Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
Drafters of this standard. Chen Zihong, Hou Liping, Tang Danzhou, Zhang Sichuan, Sun Wei.
This standard was first published.
People's Republic of China entry and exit inspection and quarantine industry standards
Coliform and E. coli counts in imported and exported foods
range
This standard is only applicable to import and export instant noodles, puffed food, mineral water, single crystal sugar, beverage, pepper, milk (requires protease treatment)
The provisions contained in the following standards constitute provisions of this standard by reference in this standard. At the time of publication of this standard, the versions shown are
To be effective. All standards will be revised and parties using this standard should explore the possibility of using the latest version of the following standards.
SN 0330-1994 General rules for microbiological examination of exported foods
抽样 sampling
Execute according to SN 0330 regulations.
. Method principle
The filter was placed in a filter, and the sample was filtered. Due to the action of the filter, coliforms and E. coli remained on the surface of the membrane. Coliform in lactose
Blue colonies were produced after culturing on monacin glucuronic acid (LMG) agar plates. Glucosinol produced by culture of Escherichia coli
Degradation of the fluorescent substrate 4-methylumbelliferone-β-D-glucuronide (MUG) and release of 4-methylumbelliferone fluorescent material at a wavelength of 366 nm
MUG-positive colonies under blue ultraviolet (UV) light showed blue-white fluorescence.
. Equipment and materials
.. Filter. One set, with pre-filter.
.. Vacuum pump.
.. Long-wave ultraviolet (UV) lamp. wavelength 366nm.
.. Membrane. organic or aqueous phase, pore size 0.45μm.
.. Incubator. 36 °C ± 1 °C.
.. Water bath. 45 °C ± 1 °C.
.. Pipette. 1mL, 10mL, with scale.
.. Glass triangle bottle and jar. 500mL.
.. Test tube. 17mm × 170mm, made of glass.
.. Glass beads. 5mm in diameter.
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