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Code of practice for marine monitoring technology. Part 5: Marine ecology
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HY/T 147.5-2013
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Basic data | Standard ID | HY/T 147.5-2013 (HY/T147.5-2013) | | Description (Translated English) | Code of practice for marine monitoring technology. Part 5: Marine ecology | | Sector / Industry | Marine Industry Standard (Recommended) | | Classification of Chinese Standard | A45 | | Classification of International Standard | 07.060 | | Word Count Estimation | 149,154 | | Quoted Standard | GB 3097; GB 4789.4-2010; GB 4789.30-2010; GB 4789.38; GB/T 12763.1; GB/T 12763.6-2007; GB 17378.3; GB 17378.5; GB 17378.7-2007; GB 18668; GB 19489; marine disaster survey specifications 3: marine Ecological disaster Investigation; | | Regulation (derived from) | Industry standard filing Notice 2013 No. 8 (No. 164 overall) | | Issuing agency(ies) | State Oceanic Administration | | Summary | This standard specifies: marine life ecology sample collection, testing, analysis, data compilation methods technical requirements. This standard applies to: inshore, offshore, far from the sea waters biological and ecological monitored. | HYT147.5-2013: Code of practice for marine monitoring technology. Part 5: Marine ecology---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Code of practice for marine monitoring technology.Part 5.Marine ecology
ICS 07.060
A45
People's Republic of China Marine Industry Standard
Marine monitoring technical regulations
Part 5.Marine Ecology
Released on.2013-04-25
2013-05-01 Implementation
Issued by the State Oceanic Administration
Table of contents
Preface Ⅴ
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 Total number of planktonic viruses-dye counting method 2
5 The total number of viruses in the sediment-staining counting method 4
6 Total number of Vibrio ---plate counting method 6
7 Enterococcus---the most probable number method (MPN) and membrane filter method 6
8 Fecal coliforms---test piece method 6
9 Graded chlorophyll a---fluorescence method 11
10 Graded Primary Productivity---14C Isotope Method 15
11 Red tide dinoflagellate cysts---optical microscope method 18
12 Pico Phytoplankton---Fluorescence microscope counting method 18
13 Microplankton---microscopic individual counting method 20
14 Small plankton---microscopic individual counting method 24
15 Fish plankton---stereomicroscope counting method 24
16 Investigation of Rare and Endangered Animals---Interview and Observation Method 27
17 Coastal Wetland Plants---Field Investigation Method 30
18 Diarrheal shellfish poisoning---enzyme-linked immunosorbent assay (ELISA) 33
19 Paralytic shellfish poison---enzyme-linked immunosorbent assay (ELISA) 33
20 Paralytic shellfish poison-high performance liquid method (HPLC) 33
21 Amnestic shellfish poisoning---enzyme-linked immunosorbent assay (ELISA) 36
22 Neurogenic shellfish poison ---enzyme-linked immunosorbent assay (ELISA) 36
23 Staphylococcus aureus-test piece method 36
24 Salmonella-Microplate Kit Method 39
25 Listeria---test piece method 42
26 Fungus---test piece method 45
27 Vibrio parahaemolyticus in seawater---polymerase chain reaction (PCR) method 47
28 Vibrio vulnificus in seawater --- polymerase chain reaction (PCR) method 49
29 Vibrio fluvialis in seawater---polymerase chain reaction (PCR) method 50
30 Vibrio alginolyticus in seawater --- polymerase chain reaction (PCR) method 51
31 Vibrio harveyi in seawater --- polymerase chain reaction (PCR) method 52
32 Vibrio cholerae in seawater --- polymerase chain reaction (PCR) method 53
33 Vibrio anguillarum in seawater --- polymerase chain reaction (PCR) method 54
34 Hepatitis A virus in seawater --- reverse transcription polymerase chain reaction (RT-PCR) method 55
35 Norovirus in seawater --- reverse transcription polymerase chain reaction (RT-PCR) method 55
36 Astrovirus in seawater --- reverse transcription polymerase chain reaction (RT-PCR) method 55
37 Rotavirus in seawater---Reverse transcription polymerase chain reaction (RT-PCR) method 57
38 Adenovirus in seawater-polymerase chain reaction (PCR) method 58
39 Enterovirus in seawater --- reverse transcription polymerase chain reaction (RT-PCR) method 59
40 Shrimp Taura Virus---Reverse Transcription Polymerase Chain Reaction (RT-PCR) Method 60
41 Shrimp White Spot Syndrome Virus---polymerase chain reaction (PCR) method 60
42 Fish lymphatic cyst virus---polymerase chain reaction (PCR) method 60
43 Fish iridescent virus---polymerase chain reaction (PCR) method 61
44 Shellfish Perkinworm --- Reye's liquid thioglycollate medium culture (RFTM) method and polymerase chain reaction (PCR) method 61
45 Shellfish Monospora---polymerase chain reaction (PCR) method 61
46 Microbial molecular identification---restriction endonuclease fragment length polymorphism (RFLP) method 61
47 Microbial tracing monitoring of fecal contamination sources---repetitive extra-gene palindromic sequence polymerase chain reaction (REP-PCR) method 64
48 Molecular identification of red tide algae species---Fluorescence in situ hybridization (FISH) 68
49 Marine Pollutants Biological Toxicity Test---Luminescent Bacteria Method 70
50 Marine Pollutants Biological Toxicity Test---Algae Test Method 73
51 Marine Pollutants Biological Toxicity Test---Polychaete Test Method 76
52 Marine Pollutants Biological Toxicity Inspection---Mollusk Inspection Law 80
53 Marine Pollutants Biological Toxicity Inspection---Crustacean Inspection Law 82
54 Marine Pollutants Biological Toxicity Test---Echinoderma Test Method 85
55 Marine Pollutants Biological Toxicity Inspection---Fish Inspection Law 87
56 Marine Sediment Biological Toxicity Test---Amphipod Test Method 89
Appendix A (Normative Appendix) Record Form 96
Appendix B (informative appendix) Counting table of physiological saline, bacteria and fungi 129
Appendix C (Normative Appendix) Table of Most Probable Number (MPN) of Bacteria in Tested Samples 132
Appendix D (Normative Appendix) Plankton sample number, biomass measurement, counting 133
Appendix E (Normative Appendix) Colorimetric Chart 135
Appendix F (Normative appendix) Salmonella confirmation test flow chart 136
Appendix G (Normative Appendix) Listeria monocytogenes confirmation test procedure 137
Appendix H (informative appendix) PCR method to detect 7 kinds of Vibrio electrophoresis simulation diagram 138
Appendix I (informative appendix) PCR, RT-PCR method to detect 10 kinds of virus electrophoresis simulation diagram 139
Appendix J (informative appendix) Test organisms---Japanese big chela 140
Figure 1 Detection procedure of direct counting method for fecal coliform test piece 8
Figure 2 MPN counting method detection procedure of fecal coliform test piece 10
Figure 3 The age structure model of Amphioxus fig 30
Figure 4 Direct counting method test procedure for Staphylococcus aureus test piece 37
Figure 5 MPN counting method test procedure for Staphylococcus aureus test piece 39
Figure 6 Process of rapid detection of Salmonella by microplate kit method 40
Figure 7 Test procedure for Listeria monocytogenes test piece 44
Figure 8 Inspection procedures for mold and yeast 46
Figure E.1 Interpretation of the results of the Salmonella microplate kit detection color chart 135
Figure F.1 Salmonella confirmation test flow chart 136
Figure G.1 Listeria monocytogenes confirmation test process 137
Figure H.1 PCR method to detect 7 kinds of Vibrio electrophoresis simulation diagram 138
Figure I.1 The electrophoresis simulation diagram of 10 viruses detected by PCR and RT-PCR methods 139
Table 1 Water sample collection level 11
Table 2 Specifications and applicable objects of various nets 25
Table 3 Plant community sample plot description survey form 31
Table 4 Reference Table 35 for the detection limits of various paralytic shellfish poisons
Table 5 Probes used in fluorescence in situ hybridization test 69
Table 6 The dilution factor of mercury chloride working solution 71
Table A.1 Plankton viruses and picoplankton marine sampling records 96
Table A.2 The direct count record of planktonic or sediment viruses 97
Table A.3 Chlorophyll sampling record table 98
Table A.4 Chlorophyll (Extraction Fluorescence Method) Measurement Record Table 99
Table A.5 Primary Productivity Granularity Classification Sampling, Filtration, and Measurement Record Table 100
Table A.6 Record of the number of picophytoplankton cells 101
Table A.7 Marine Sampling Record of Microplankton 102
Table A.8 Microplankton sample registration form 103
Table A.9 Water sample plankton number count record table 104
Table A.10 Number of micro-plankton specimens counted records 105
Table A.11 Statistics on the number of microplankton 106
Table A.12 List of microplankton species 107
Table A.13 Marine Collection Record of Fish Plankton 108
Table A.14 Fish plankton specimen registration form 109
Table A.15 Fish plankton count record table 110
Table A.16 Statistics of fish plankton quantity 111
Table A.17 Marine Rare and Endangered Swimming Animal Observation Record Table 112
Table A.18 Statistics of observation results of marine rare and endangered swimming animals 112
Table A.19 Observation records of rare and endangered sea birds sample points Table 113
Table A.20 Observation Records of Rare and Endangered Sea Bird Transects Table 113
Table A.21 Wenchang fish field sampling record 114
Table A.22 Indoor analysis record of Amphioxus population structure 114
Table A.23 Investigation record of coastal wetland plants 115
Table A.24 Test record of pathogenic Vibrio in seawater 116
Table A.25 Marine Virus Record Form 117
Table A.26 Fish lymphatic cyst virus detection record table 118
Table A.27 Biological toxicity test site sampling record table 119
Table A.28 Luminescent bacteria acute toxicity test record table 120
Table A.29 Luminescent bacteria method to determine environmental toxicity classification standards 120
Table A.30 Algae toxicity test record table 121
Table A.31 Percentage and probability unit conversion table 122
Table A.32 Algae toxicity test data statistical conversion registration form 123
Table A.33 Marine biological toxicity test record table 124
Table A.34 Marine biological toxicity test data statistical conversion registration form 125
Table A.35 Amphipod toxicity test biological result record table 126
Table A.36 Amphipod toxicity test physical result record table 127
Table A.37 Amphipod toxicity test biological results statistics table 128
Table B.1 Direct count of bacteria (fungus) test piece 130
Table B.2 Bacteria test piece MPN count table 131
Table C.1 The most probable number (MPN) of bacteria in the tested sample Table 132
Foreword
HY/T 147 "Marine Monitoring Technical Regulations" is divided into seven parts.
---Part 1.Sea water;
---Part 2.Sediment;
---Part 3.Organisms;
---Part 4.Ocean atmosphere;
---Part 5.Marine ecology;
---Part 6.Marine Hydrology, Meteorology and Sea Ice;
---Part 7.Satellite remote sensing technology methods.
This part is part 5 of HY/T 147.
This section was drafted in accordance with the rules given in GB/T 1.1-2009.
This part was proposed by the National Marine Environmental Monitoring Center.
This part is under the jurisdiction of the National Marine Standardization Technical Committee (SAC/TC283).
Drafting organizations of this section. National Marine Environmental Monitoring Center, South China Sea Environmental Monitoring Center of State Oceanic Administration, East China Sea Environmental Monitoring Center of State Oceanic Administration
Monitoring Center, North Sea Environmental Monitoring Center of State Oceanic Administration.
The main drafters of this section. Han Gengchen, Fan Jingfeng, Ma Yongan, Jiang Wenbo, Zhang Zhendong, Lin Fengao, Li Hongbo, Liang Bin, Xu Daoyan, Shao Kuishuang,
Li Dongmei, Liu Yongjian, Liu Shuxi, Yuan Xiutang, Yan Qilun, Liu Guize, Wang Lijun, Liu Changan, Feng Zhiquan, Wang Zhenliang, Liu Na, Yu Zhanguo, Huang Chuguang,
Gao Yang, Li Xiuqin, Wei Guiqiu, Yi Bin, Xiao Yuzhang, Chen Jiahui, Li Haitao, Xiong Xiaofei, Dong Yanhong, Wu Shiwei, Lu Chuqian, Cheng Xiangsheng, Liu Caicai,
Sun Yawei, Qin Yutao, Li Yiyun, Xia Yongjian, Fan Lijing, Ji Huanhong, Huang Hui, Gao Junzhang, Xin Dinghao, Song Chenyao, Cui Wenlin, Qi Yanping, Zhang Qi,
Wang Taisen, Wang Xiaoqing, Li Qinliang, Zhao Sheng, Sun Beibei, Zhang Qingbo.
Marine monitoring technical regulations
Part 5.Marine Ecology
1 Scope
This part of HY/T 147 specifies the technical requirements for the methods of sampling, testing, analysis, and data compilation of marine biological ecology.
This part is applicable to the monitoring of biological ecology in nearshore, offshore and far sea areas.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB 3097 Sea Water Quality Standard
GB 4789.4-2010 National Food Safety Standard Food Microbiological Inspection Salmonella Inspection
GB 4789.30-2010 National Food Safety Standard Food Microbiological Examination Examination of Listeria Monocytogenes
GB 4789.38 National Food Safety Standard, Food Microbiological Inspection, Count of Escherichia Coli
GB/T 12763.1 Marine Survey Specification Part 1.General
GB/T 12763.6-2007 Marine Survey Specification Part 6.Marine Biological Survey
GB 17378.3 Marine Monitoring Specification Part 3.Sample Collection, Storage and Transportation
GB 17378.5 Marine Monitoring Specification Part 5.Sediment Analysis
GB 17378.7-2007 Marine Monitoring Specification Part 7.Offshore Pollution Ecological Investigation and Biological Monitoring
GB 18668 Marine sediment quality
GB 19489 Laboratory Biosafety General Requirements
Technical Specifications for Marine Disaster Investigation Part 3.Marine Ecological Disaster Investigation
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Plankton plankton
A creature that lacks developed motor organs, has no or only weak motor ability, is suspended in the water layer, and often moves with water. Including float
There are two major categories of zooplankton and zooplankton. Plankton can be divided into the following types according to the size of the individual. those with a particle size of less than 2μm are called pico
Plankton (picoplankton); the particle size of 2μm~20μm is called nanoplankton; the particle size is 20μm~
200μm is called small plankton (microplankton or netplankton);.200μm~2000μm is called medium plankton
Organisms (mesoplankton); those with a particle size between.2000μm and 20mm are called macroplanktons; the particle size is greater than
20mm is called megaplankton. In addition, fish plankton (ichthyoplankton) are fish eggs and larvae.
[GB/T 12763.6-2007, definition 3.10]
3.2
Distance sampling distancesampling
Using bird identification technology at different distances and habitats, estimate the number of birds within a certain range or distance, and collect ecological population data.
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